@article { ISI:000231864200007, title = {In ovulo rescue of hybrid embryos in flame seedless grapes - influence of pre-bloom sprays of benzyladenine}, journal = {Scientia Horticulturae}, volume = {106}, number = {3}, year = {2005}, month = {OCT}, pages = {353-359}, publisher = {ELSEVIER SCIENCE BV}, type = {Article}, address = {PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS}, abstract = {

Based on the theory that cytokinins are known to increase the sink strength of seeds for assimilates, the present investigation was carried out to study the influence of pre-bloom and bloom time sprays of benzyladenine (BA) on embryo recovery, germination and plant development in Flame Seedless grape variety by using in ovulo rescue methods. The combined effect of pollen parent type and sprays of BA drastically improved these parameters and the embryo recovery was more than doubled in all the crosses, except in cross FS x Concord. Also, germination of embryos was higher when BA sprays were given. Recovery of embryos and growth depended on BA treatment and the pollen parent types. (c) 2005 Elsevier B.V. All rights reserved.

}, keywords = {benzyladenine, flame seedless, in ovulo embryo rescue, interspecific hybridization, Vitis spp.}, issn = {0304-4238}, doi = {10.1016/j.scienta.2005.04.002}, author = {Bharathy, P. V. and Karibasappa, G. S. and Patil, S. G. and Agrawal, Dinesh C.} } @article { ISI:000250387000005, title = {Synergistic effect of CPPU and benzyladenine on embryo rescue in six. stenospermocarpic cultivars of grapevine}, journal = {Vitis}, volume = {46}, number = {4}, year = {2007}, month = {JAN}, pages = {188-191}, publisher = {BUNDESANSTALT ZUCHTUNGS FORSCHUNG KULTURPFLANZEN}, type = {Article}, address = {INST REBENZUCHTUNG GEILWEILERHOF, D-76833 SIEBELDINGEN, GERMANY}, abstract = {

In ovulo/embryo culture technique has been used to recover hybrids from seedless grapevines. The present investigation was carried out to study the influence of pre-bloom sprays of N-(2-chloro-4-pyridyl)-N{\textquoteright}-phenylurea (CPPU) - a synthetic cytokinin, and N-6-benzyladenine (BA) supplemented in culture media on embryo recovery in six stenospermocarpic grapevine cultivars. The results showed synergistic effect of CPPU and BA on embryo recovery. Though a CPPU spray alone increased embryo recovery in four out of six cultivars tested, the efficiency was enhanced several fold on culture of excised ovules on media supplemented with BA. The percentage of embryo recovery also depended on BA concentration and varied among six cultivars indicating a genotypic influence as well. Germination percentage of rescued embryos varied among the six cultivars and mostly corresponded with embryo recovery. Germinated embryos developed into normal plantlets. Present study demonstrates that spraying of panicles with CPPU and incorporation of BA in the ovule culture medium can enhance the embryo recovery in stenospermocarpic cultivars of grapevine.

}, keywords = {benzyladenine, CPPU, embryo rescue, seedless grapes}, issn = {0042-7500}, author = {Nookaraju, A. and Barreto, M. S. and Karibasappa, G. S. and Agrawal, Dinesh C.} } @conference { ISI:000257237400015, title = {Variability among vitis vinifera cultivars to in vitro propagation}, booktitle = {Proceedings of the International Symposium on Grape Production and Processing}, series = {ACTA HORTICULTURAE}, number = {785}, year = {2008}, note = {International Symposium on Grape Production and Processing, Baramati, INDIA, FEB 06-11, 2006}, month = {MAY}, pages = {127-139}, publisher = {Agr \& Processed Food Prod Export Dev Author; Indian Farmers Fertilizer Cooperat Ltd; Maharashtra Agro Industries Dev Corp Ltd; Indian Council Agr Res; Natl Res Ctr Grapes; Federat Indian Chambers Commerce \& Ind; Natl Hort Board; Minist Food Proc Industrie}, organization = {Agr \& Processed Food Prod Export Dev Author; Indian Farmers Fertilizer Cooperat Ltd; Maharashtra Agro Industries Dev Corp Ltd; Indian Council Agr Res; Natl Res Ctr Grapes; Federat Indian Chambers Commerce \& Ind; Natl Hort Board; Minist Food Proc Industrie}, address = {Po Box 500, 3001 Leuven 1, Belgium}, abstract = {

Response of grapevines to tissue culture has been reported to be genotype dependent, hence it becomes imperative to optimize culture conditions for newly developed varieties or cultivars, needing a large scale planting but availability of sufficient planting stock is a constraint. In the present study, conditions for in vitro propagation of six popular table grape cultivars have been standardized. Single node stem segments of cultivars 2A-Clone of Thompson Seedless, Red Globe, Crimson Seedless, Thompson Seedless, Flame Seedless and Italia cultured on ten different basal media showed varied percentages of bud break and morphogenetic responses. The percentage of bud break varied among the six cultivars. Direct rooting at basal ends of single node segments was observed in all the cultivars except in Italia. Woody plant medium induced the highest response and Eriksson medium the least in all the cultivars tested. Rooted nodal segments with shoots in axils could be established into whole plants on potting. Six cultivars showed different optimum concentrations of growth regulators for induction of maximum number of multiple shoots in both primary as well as secondary nodal segments. Shoot proliferation could be enhanced by several fold in the majority of cultivars on culture of initial shoot clumps to glass bottles instead of culture tubes. Six cultivars showed significant differences in optimum requirement of nutrients and growth regulators for shoot elongation, in vitro and ex vitro rooting and survival rate. Tissue culture plants of all the six cultivars could establish successfully in a greenhouse.

}, keywords = {basal media, Grapevine, growth regulators, micropropagation, Tissue culture, Vitis}, isbn = {978-90-6605-268-0}, issn = {0567-7572}, author = {Barreto, M. S. and Nookaraju, A. and Joglekar, A. M. and Karibasappa, G. S. and Agrawal, Dinesh C.}, editor = {Adsule, P. G. and Sawant, I. S. and Shikhamany, S. D.} }