@article { ISI:000331347300019, title = {Culturable bacterial phylogeny from a shallow water hydrothermal vent of espalamaca (Faial, Azores) reveals a variety of novel taxa}, journal = {Current Science}, volume = {106}, number = {1}, year = {2014}, month = {JAN}, pages = {58-69}, publisher = {INDIAN ACAD SCIENCES}, type = {Article}, address = {C V RAMAN AVENUE, SADASHIVANAGAR, P B \#8005, BANGALORE 560 080, INDIA}, abstract = {

Phylogenetic diversity of the 16S rRNA gene associated with the domain bacteria was examined at the level of operational taxonomic units (OTUs) using the rarefaction analysis from a newly identified shallow water hydrothermal vent, Espalamaca in the Azorean Island Faial (Horta), North Atlantic Ocean. Diluted sea water nutrient agar amended with metals manganese, lead, iron and element sulphur, could help in retrieving highest OTUs (95) from the vent and 39 OTUs from nonvent. Molecular tools implemented on bacterial census indicated the occupancy of gamma-Proteobacteria by 55.78\%, alpha-Proteobacteria by 21.05\% and 12.63\% of Bacteroidetes in the total population. Occurrence of novel species maximized with alpha-Proteobacteria (11/20) followed by Bacteroidetes (5/12) signified the necessity of studying these groups to strengthen the biodiversity database. Shannon index (H{\textquoteright}) and the Chao I richness estimator illustrated a strong bacterial community in the venting area. The current study confers many bacterial genera which were not reported earlier in any of the shallow water vents and adds 33 new taxa to the database.

}, keywords = {Bacterial phylogeny, metals, novel taxa, rarefaction analysis, shallow vent}, issn = {0011-3891}, author = {Rajasabapathy, Raju and Mohandass, Chellandi and Colaco, Ana and Dastager, Syed Gulam and Santos, Ricardo Serrao and Meena, Ram Murti} } @article { ISI:000364504500016, title = {Citreicella manganoxidans sp nov., a novel manganese oxidizing bacterium isolated from a shallow water hydrothermal vent in Espalamaca (Azores)}, journal = {Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology}, volume = {108}, number = {6}, year = {2015}, month = {DEC}, pages = {1433-1439}, publisher = {SPRINGER}, address = {VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS}, abstract = {

A Gram-stain negative, non-motile, non-spore forming, aerobic and rod or narrow lemon-shaped bacterial strain, VSW210(T), was isolated from surface seawater in a shallow water hydrothermal vent region in Espalamaca (Azores). Strain VSW210(T) was found to grow optimally at 30 A degrees C, at pH 7 and in the presence of 2-6 \% (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain VSW210(T) clusters with the type strain Citreicella marina CK-I3-6(T) (sequence similarity value of 99.6 \%), but DNA-DNA hybridization showed DNA-DNA relatedness between the strain VSW210(T) and C. marina CK-I3-6(T) to be 55.8 +/- A 3.2 \%. The DNA G+C content of strain VSW210(T) was determined to be 67.4 mol\%. The cellular fatty acid profiles of strain VSW210(T) was found to contain C-18:1 omega 7c (80.1 \%) and C-16:0 (9.2 \%). The major polar lipids in strain VSW210(T) were identified as phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unidentified phospholipid. Strain VSW210(T) was found to be able to oxidize soluble Mn(II) to insoluble MnO2, which was confirmed with LBB staining. Differential phenotypic properties and genetic uniqueness revealed that this strain VSW210(T) is distinguishable from other species of the genus Citreicella. On the basis of the data presented, strain VSW210(T) is considered to represent a novel species of the genus Citreicella, for which the name Citreicella manganoxidans sp. nov. is proposed. The type strain is VSW210(T) (=KCTC 32497(T) = MCC 2286(T)).

}, keywords = {Azores, Citreicella manganoxidans sp nov, Hydrothermal vent, Polyphasic taxonomy, Seawater}, issn = {0003-6072}, doi = {10.1007/s10482-015-0597-x}, author = {Rajasabapathy, Raju and Mohandass, Chellandi and Dastager, Syed Gulam and Liu, Qing and Li, Wen-Jun and Colaco, Ana} } @article {43031, title = {Complete metagenome sequencing based bacterial diversity and functional insights from basaltic hot spring of Unkeshwar, Maharashtra, India}, journal = {Genomics Data}, volume = {7}, year = {2016}, month = {MAR}, pages = {140-143}, abstract = {

Unkeshwar hot springs are located at geographical South East Deccan Continental basalt of India. Here, we report the microbial community analysis of this hot spring using whole metagenome shotgun sequencing approach. The analysis revealed a total of 848,096 reads with 212.87Mbps with 50.87\% G+C content. Metagenomic sequences were deposited in SRA database with accession number (SUB1242219). Community analysis revealed 99.98\% sequences belonging to bacteria and 0.01\% to archaea and 0.01\% to Viruses. The data obtained revealed 41 phyla including bacteria and Archaea and including 719 different species. In taxonomic analysis, the dominant phyla were found as, Actinobacteria (56\%), Verrucomicrobia (24\%), Bacteriodes (13\%), Deinococcus-Thermus (3\%) and firmicutes (2\%) and Viruses (2\%). Furthermore, functional annotation using pathway information revealed dynamic potential of hot spring community in terms of metabolism, environmental information processing, cellular processes and other important aspects. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of each contig sequence by assigning KEGG Orthology (KO) numbers revealed contig sequences that were assigned to metabolism, organismal system, Environmental Information Processing, cellular processes and human diseases with some unclassified sequences. The Unkeshwar hot springs offer rich phylogenetic diversity and metabolic potential for biotechnological applications.

}, doi = {10.1016/j.gdata.2015.12.031}, author = {Mehetre, Gajanan T. and Paranjpe, Aditi S. and Dastager, Syed Gulam and Dharne, Mahesh S.} }