@article { ISI:000323497900010, title = {Alishewanella solinquinati sp nov., isolated from soil contaminated with textile dyes}, journal = {Current Microbiology}, volume = {67}, number = {4}, year = {2013}, month = {OCT}, pages = {454-459}, publisher = {SPRINGER}, address = {233 SPRING ST, NEW YORK, NY 10013 USA}, abstract = {

A novel Gram-negative, motile, rod-shaped, facultative anaerobic bacterial strain, KMK6(T), was isolated from soil contaminated with textile dyes from an industrial estate located at Ichalkaranji, Maharashtra, India, and its taxonomical position was established by using a polyphasic approach. The major cellular fatty acids included C-17:1 omega 8c, summed feature 3 (C-16:1 omega 7c and/or iso-C-15:0 2-OH), C-17:0, C-16:0,C- and C-18:1 omega 7c. The DNA G+C content of strain KMK6(T) was 48.8 mol \%. 16S rRNA gene sequence analysis confirmed its placement in the genus Alishewanella, and exhibited sequence similarity levels of below 97 \% to the type strains of validly published Alishewanella species. On the basis of genotypic and phenotypic evidence, strains KMK6(T) is considered to be a novel species of the genus Alishewanella, for which we propose that strain KMK6(T) (=NCIM 5295(T) =BCRC 17848(T)) is assigned to a novel species, Alishewanella solinquinati sp. nov.

}, issn = {0343-8651}, doi = {10.1007/s00284-013-0385-7}, author = {Kolekar, Yogesh M. and Pawar, Shrikant P. and Adav, Sunil S. and Zheng, Liu-Qiang and Li, Wen-Jun and Shouche, Yogesh S. and Dastager, Syed Gulam and Kodam, Kisan M.} } @article { ISI:000344448000015, title = {Anti-fungal potentials of extracellular metabolites of western ghats isolated streptomyces sp NII 1006 against moulds and yeasts}, journal = {Indian Journal of Experimental Biology }, volume = {52}, number = {11}, year = {2014}, month = {NOV}, pages = {1138-1146}, publisher = {NATL INST SCIENCE COMMUNICATION-NISCAIR}, type = {Article}, address = {DR K S KRISHNAN MARG, PUSA CAMPUS, NEW DELHI 110 012, INDIA}, abstract = {

Realization of hazardious effects of chemical fungicides has led to an interest in the usage of biocontrol agents. The present study, therefore, evaluates the biocontrol efficacy of Western Ghats (India) soil bacterial isolates. A potential strain NII 1006 was evaluated for its antagonistic property against a diverse range of moulds and yeasts. The strain was characterized morphologically, biochemically and molecularly, which revealed the isolate belonged to Streptomyces genus. Organic solvent extracts of NII 1006 culture filtrates inhibited the growth of the test pathogens indicating that growth suppression was due to extracellular anti-fungal metabolites present in the culture filtrates. The strain produced extracellular chitinase enzyme in addition to some stable partially purified anti-fungal compounds. Morphological changes such as hyphae degradation into debris and abnormal shapes were observed in test fungi and yeast grown on potato dextrose broth that contained the NII 1006 culture filtrate. The cell free supernatant has a tolerance to wide range of pH, temperature and enzymes such as lipase and protease. The biocontrol potential of NII 1006 strain may be correlated significantly with their ability to produce antibiotics as well as extracellular hydrolytic enzymes particularly chitinolytic enzyme.

}, keywords = {Anti-fungal activity, Biocontrol, Extracellular anti-fungal metabolites, Streptomyces}, issn = {0019-5189}, author = {Jayamurthy, Himani and Sajna, Kuttavan Valappil and Dastager, Syed Gulam and Pandey, Ashok} } @article { ISI:000343905600003, title = {Arthrobacter enclensis sp. nov., isolated from sediment sample}, journal = {Archives of Microbiology}, volume = {196}, number = {11}, year = {2014}, month = {NOV}, pages = {775-782}, publisher = {SPRINGER}, type = {Article}, address = {233 SPRING ST, NEW YORK, NY 10013 USA}, abstract = {

A novel bacterial strain designated as NIO-1008(T) was isolated from marine sediments sample in Chorao Island India. Cells of the strains were gram positive and non-motile, displayed a rod-coccus life cycle and formed cream to light grey colonies on nutrient agar. Strain NIO-1008(T) had the chemotaxonomic markers that were consistent for classification in the genus Arthrobacter, i.e. MK-9(H-2) (50.3 \%), as the major menaquinone, and the minor amount of MK-7 (H-2-27.5 \%), MK-8 (H-4-11.6 \%) and MK-8 (H-2-10.4 \%). anteiso-C-15:0, iso-C-15:0, iso-C-16:0 and C-15:0 were the predominant fatty acids. Galactose, glucose and rhamnose are the cell-wall sugars, and DNA G+C content was 61.3 mol\%. Phylogenetic analysis, based on 16S rRNA gene sequencing, showed that the strains were most similar to Arthrobacter equi IMMIB L-1606(T), Arthrobacter chlorophenolicus DSM 12829(T), Arthrobacter defluvii KCTC 19209(T) and Arthrobacter niigatensis CCTCC AB 206012(T) with 98.5, 98.4, 98.0 and 97.8 \%, respectively, and formed a separate lineage. Combined phenotypic data and DNA-DNA hybridization data supported the conclusion that strains NIO-1008(T) represent a novel species within the genus Arthrobacter, for which the name Arthrobacter enclensis sp. nov., is proposed. The type strain is NIO-1008(T) = (NCIM 5488(T) = DSM 25279(T)).

}, keywords = {Arthrobacter, Chorao Island, Marine sediment, Polyphasic}, issn = {0302-8933}, doi = {10.1007/s00203-014-1016-9}, author = {Dastager, Syed Gulam and Qin, Liu and Tang, Shan-Kun and Krishnamurthi, Srinivasan and Lee, Jae-Chan and Li, Wen-Jun} } @article { ISI:000343905600004, title = {Arthrobacter enclensis sp. nov., isolated from sediment sample (vol 196, pg 775, 2014)}, journal = {Archives of Microbiology}, volume = {196}, number = {11}, year = {2014}, month = {NOV}, pages = {783}, publisher = {SPRINGER}, type = {Correction}, address = {233 SPRING ST, NEW YORK, NY 10013 USA}, issn = {0302-8933}, doi = {10.1007/s00203-014-1041-8}, author = {Dastager, Syed Gulam and Liu, Qing and Tang, Shan-Kun and Krishnamurthi, Srinivasan and Lee, Jae-Chan and Li, Wen-Jun} } @article { ISI:000372335600061, title = {Actinorectispora indica gen. nov., sp nov isolated from soil, a member of the family pseudonocardiaceae}, journal = {International Journal of Systematic and Evolutionary Microbiology}, volume = {66}, number = {2}, year = {2016}, month = {FEB}, pages = {939-945}, publisher = {SOC GENERAL MICROBIOLOGY}, address = {MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLAND}, abstract = {

The taxonomic positions of three Gram-stain-positive, aerobic strains, designated YIM 75722, 75726 and 75728(T), and isolated from a soil sample collected from Kurnool of Andhra Pradesh province, India, were assessed using a polyphasic approach. Growth was observed at pH 7.0-10.0 (optimum pH 7.0), 15-28 degrees C (optimum 28 degrees C) and 0-8\% (w/v) NaCl (grew without NaCl). Strains showed cylindrical spores with straight-chain morphology on aerial mycelium, but did not reveal sporangium-like structures or fragmentation of the substrate mycelium. Whole-cell hydrolysates of all strains contained galactose and ribose as the diagnostic sugars and meso-diaminopimelic acid as the diamino acid. The predominant menaquinone was MK-9(H-4); MK-9 (H-6) and MK-10 (H-4) were present in smaller amounts. The phospholipid pattern consisted mainly of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylcholine. The major fatty acids were i-C-15: 0, ai-C-15: 0, i-C-17 : 0 and ai-C-17 : 0. The genomic DNA G+C content was 68.0 mol\%. Phylogenetic analysis, based on 16S rRNA gene sequences, revealed that strain YIM 75728(T) should be placed within the family Pseudonocardiaceae, in which the strain formed a distinct lineage. The combination of phylogenetic analysis, phenotypic characteristics and chemotaxonomic data support the conclusion that strain YIM 75728(T) represents a novel species of a novel genus of the family Pseudonocardiaceae for which the name Actinorectispora indica gen. nov., sp. nov., is proposed. Strain YIM 75728(T) (=DSM 45410(T)=CCTCC AA 209065(T)) is the type strain of Actinorectispora indica. Strain YIM 75728(T) was considered as the type strain over the other two strains based on the highest sequence read length of the strain.

}, issn = {1466-5026}, doi = {10.1099/ijsem.0.000814}, author = {Quadri, Syed Raziuddin and Tian, Xin-Peng and Zhang, Jing and Al Ruwaili, Jamal and Hozzein, Wael N. and Agsar, Dayanand and Li, Wen-Jun and Dastager, Syed Gulam} } @article { ISI:000377464900011, title = {Antioxidative metabolites synthesized by marine pigmented vibrio sp and its protection on oxidative deterioration of membrane lipids}, journal = {Applied Biochemistry and Biotechnology}, volume = {179}, number = {1}, year = {2016}, month = {APR}, pages = {155-167}, publisher = {HUMANA PRESS INC}, address = {999 RIVERVIEW DRIVE SUITE 208, TOTOWA, NJ 07512 USA}, abstract = {

Bacterial strain Vibrio sp. (PIGB 184) isolated from water samples of the Arabian Sea and identified through 16S rRNA demonstrated the production of pigmentary antioxidants with higher ABTS activities 90.9 +/- 0.42 \% in comparison with the standard commercial pigmented antioxidant, quercetin 88.8 +/- 1.4 \%. Antioxidative metabolites of this strain substantially inhibit the lipid peroxidation (LPO) reactions tested in sheep liver and brain. The antioxidant compounds produced by the Vibrio sp. (PIGB 184), analysed by GC-MS, reveals that it is composed mostly of phenol, 2,4-bis(1,1-dimethylethyl) and pyrrolo[1,2-a]pyrazine-1,4-dione,hexahydro-3-(2-methylpropyl). The interrelationship assessed between LPO and the phenolic compounds showed significant correlation with anti-LPO properties (R (2) = 0.9698 to 0.9861). These compounds are responsible for obstruction of harmful radical associated biochemical reactions in biological systems. Pigmented metabolites also tested for attributive biological properties against pathogenic bacteria showed prominent inhibition towards Gram-positive organisms (31.25 to 62.5 mu g ml(-1)). From this study, it may be suggested that the marine bacterium PIGB 184 could be used as a potential bio-resource for antioxidants and needs to be worked out for mass production.

}, keywords = {Antioxidants, Lipid peroxidation, Marine pigmented bacteria, Pathogen inhibition, Phenolics}, issn = {0273-2289}, doi = {10.1007/s12010-016-1985-z}, author = {Pawar, Ravindra and Mohandass, Chellandi and Dastager, Syed Gulam and Kolekar, Yogesh M. and Malwankar, Rahul} } @article {43933, title = {Anti-microbial activity of chrysomycin a produced by streptomyces sp. against mycobacterium tuberculosis}, journal = {RSC Advances}, volume = {7}, year = {2017}, month = {JUL}, pages = {36335-36339}, type = {Article}, abstract = {Limited efficacy of the BCG (Bacillus Calmette{\textendash}Gu{\'e}rin) vaccine against adult tuberculosis and the emergence of resistance to existing anti-tuberculosis drugs compel discovery of novel antibiotics against Mycobacterium tuberculosis. Actinomycetes are still an attractive platform for the discovery of new antimicrobials, especially from untapped natural hotspots, despite the belief that they are an exhausted resource after repeated re-discoveries. Herein we report the isolation and identification of chrysomycin A from an actinomycete isolated from a coastal area in Kerala. We show for the first time that it has antimycobacterial activity. It was found to be bactericidal to planktonic and intracellular M. tuberculosis with an MIC of 3.125 μg mL$^{-}${\textonesuperior}; it is non-hemolytic and has negligible cytotoxicity. The actinomycete that produces chrysomycin A was found to be a Streptomyces sp. through 16S rRNA gene sequencing.}, doi = {10.1039/C7RA05576E}, author = {Balaji, Muralikrishnan and Dan, Vipin Mohan and Joseph, Vinodh and Jamsheena, Vellekkatt and Ramachandran, Ranjit and Thomas, Sabu and Dastager, Syed Gulam and Kumar, K. Santhosh and Lankalapalli, Ravi Shankar and Kumar, R. Ajay} }