@article { ISI:000344448000015,
	title = {Anti-fungal potentials of extracellular metabolites of western ghats isolated streptomyces sp NII 1006 against moulds and yeasts},
	journal = {Indian Journal of Experimental Biology },
	volume = {52},
	number = {11},
	year = {2014},
	month = {NOV},
	pages = {1138-1146},
	publisher = {NATL INST SCIENCE COMMUNICATION-NISCAIR},
	type = {Article},
	address = {DR K S KRISHNAN MARG, PUSA CAMPUS, NEW DELHI 110 012, INDIA},
	abstract = {<p>Realization of hazardious effects of chemical fungicides has led to an interest in the usage of biocontrol agents. The present study, therefore, evaluates the biocontrol efficacy of Western Ghats (India) soil bacterial isolates. A potential strain NII 1006 was evaluated for its antagonistic property against a diverse range of moulds and yeasts. The strain was characterized morphologically, biochemically and molecularly, which revealed the isolate belonged to Streptomyces genus. Organic solvent extracts of NII 1006 culture filtrates inhibited the growth of the test pathogens indicating that growth suppression was due to extracellular anti-fungal metabolites present in the culture filtrates. The strain produced extracellular chitinase enzyme in addition to some stable partially purified anti-fungal compounds. Morphological changes such as hyphae degradation into debris and abnormal shapes were observed in test fungi and yeast grown on potato dextrose broth that contained the NII 1006 culture filtrate. The cell free supernatant has a tolerance to wide range of pH, temperature and enzymes such as lipase and protease. The biocontrol potential of NII 1006 strain may be correlated significantly with their ability to produce antibiotics as well as extracellular hydrolytic enzymes particularly chitinolytic enzyme.</p>},
	keywords = {Anti-fungal activity, Biocontrol, Extracellular anti-fungal metabolites, Streptomyces},
	issn = {0019-5189},
	author = {Jayamurthy, Himani and Sajna, Kuttavan Valappil and Dastager, Syed Gulam and Pandey, Ashok}
}
@article { ISI:000344448000007,
	title = {Development of bioconjugate from streptomyces tyrosinase and gold nanoparticles for rapid detection of phenol constituents},
	journal = {Indian Journal of Experimental Biology},
	volume = {52},
	number = {11},
	year = {2014},
	month = {NOV},
	pages = {1071-1081},
	publisher = {NATL INST SCIENCE COMMUNICATION-NISCAIR},
	type = {Article},
	address = {DR K S KRISHNAN MARG, PUSA CAMPUS, NEW DELHI 110 012, INDIA},
	abstract = {<p>Most of the phenol compounds are toxic and have been considered as hazardous pollutants. Several physicochemical and biological methods are available to detect and monitor the phenol pollutants in water and soil. In the present study, phenol constituents of winery, paper and plastic industrial effluents were successfully detected employing tyrosinase-gold nanoparticles bioconjugate. The synthesis of extracellular tyrosinase and gold nanoparticles was achieved by a single isolate of Streptomyces sp. DBZ-39. Enhanced production (369.41 IU) of tyrosinase was produced in submerged bioprocess employing response surface method with central composite design. Extracellular gold nanoparticles synthesized (12-18 nm) by Streptomyces sp. DBZ-39 were characterized with TEM, EDAX and FTIR analysis. A rapid detection (within 10 min) of phenol constituents from winery effluents was achieved by bioconjugate, when compared to tyrosinases and gold nanoparticles independently. Streptomyces tyrosinase could exhibit relatively a better performance than commercially available mushroom tyrosinase in the detection of phenol constituents. Winery effluent has shown much higher content (0.98 O.D) of phenol constituents than paper and plastic effluents based on the intensity of color and U.V absorption spectra.</p>},
	keywords = {Bioconjugate, gold nanoparticles, Phenol detection, Streptomyces, Tyrosinase},
	issn = {0019-5189},
	author = {Zainab, Mazhari Bi Bi and Madhusudhan, D. N. and Raghavendra, H. and Dastager, Syed Gulam and Dayanand, Agsar}
}
