@article { ISI:000253497900006,
	title = {Cellular polyamines influence maturation and germination of somatic embryos from pro-embryonal masses of two grapevine cultivars},
	journal = {Vitis},
	volume = {47},
	number = {1},
	year = {2008},
	month = {JAN},
	pages = {31-34},
	publisher = {BUNDESANSTALT ZUCHTUNGS FORSCHUNG KULTURPFLANZEN},
	type = {Article},
	address = {INST REBENZUCHTUNG GEILWEILERHOF, D-76833 SIEBELDINGEN, GERMANY},
	abstract = {<p>Somatic embryos of grapevine multiply repeatedly and often fail to mature and germinate due to factors like dormancy and embryo teratology. The present investigation was carried out to improve the maturation and germination of somatic embryos from pro-embryonal masses (PEM) of 2A-Clone and {\textquoteleft}Crimson Seedless{\textquoteright}, two seedless grapevine cultivars. Also, the aim was to study a correlation between cellular and residual polyamine (PA) levels in PEM and culture media. The efficiency of maturation and germination of embryos from PEM varied significantly between the two cultivars and depended on incubation period and type of PAs in the medium. HPLC analysis showed that higher levels of cellular putrescine in PEM had correlation with maturation and germination percentages in both cultivars. The levels of three PAs depleted in the media rapidly indicating its uptake by PEM. Of the three PAs, putrescine (PUT) was the most effective and resulted in 100.0 or 92.0 \% maturation at 14 d or 30 d in 2A-Clone or {\textquoteleft}Crimson Seedless{\textquoteright}, respectively. The maximum germination of somatic embryos was recorded with PUT at 14 d or 21 d in 2A-Clone or {\textquoteleft}Crimson Seedless{\textquoteright}, respectively.</p>},
	keywords = {germination, Grapevine, maturation, polyamines, {\textquoteleft}2A-Clone{\textquoteright}, {\textquoteleft}Crimson Seedless{\textquoteright}},
	issn = {0042-7500},
	author = {Nookapaju, A. and Barreto, M. S. and Agrawal, Dinesh C.}
}
@article { ISI:000367464000006,
	title = {c Squalene synthase gene from medicinal herb bacopa monniera: molecular characterization, differential expression, comparative modeling, and docking studies},
	journal = {Plant Molecular Biology Reporter},
	volume = {33},
	number = {6},
	year = {2015},
	month = {DEC},
	pages = {1675-1685},
	publisher = {SPRINGER},
	address = {233 SPRING ST, NEW YORK, NY 10013 USA},
	abstract = {<p>The widespread pharmaceutically important triterpenoid saponins are synthesized via isoprenoid pathway. The formation of squalene is the key regulatory point in triterpene biosynthesis, catalyzed by squalene synthase (SQS). The present study deals with the detailed characterization of SQS by molecular, biochemical, and computational means from Bacopa monniera, an immensely important medicinal plant rich in triterpenoid saponin, bacosides. A full-length SQS gene was isolated from B. monniera, characterized as B. monniera squalene synthase (BmSQS) (1242 bp) encoding 414 amino acids. Deduced amino acid sequence of BmSQS showed highly conserved consensus aspartate-rich motifs (DXXXD) and catalytic site residues. Phylogenetic analysis showed that BmSQS belongs to dicot group having closest relationship with Salvia miltiorrhiza. Semiquantitative and real-time PCR studies showed that the BmSQS messenger RNA (mRNA) expression level was higher in vegetative parts (roots) as compared to floral parts. Methyl jasmonate induces the BmSQS mRNA expression in all tissues tested, while salicylic acid, cold, and salt induce much higher expression in roots. Homology modeling and docking simulations of BmSQS showed the pivotal roles of Asp77, Asp81, Asp213, Asp217, and Tyr168 in catalysis.</p>},
	keywords = {Abiotic stress, Bacopa monniera, Comparative modeling and docking, Differential expression, Isoprenoid pathway, Squalene synthase},
	issn = {0735-9640},
	doi = {10.1007/s11105-015-0864-z},
	author = {Vishwakarma, Rishi K. and Patel, Krunal and Sonawane, Prashant and Kumari, Uma and Singh, Somesh and Ruby and Shakeel Abbassi and Agrawal, Dinesh C. and Tsay, Hsin-Sheng and Khan, Bashir Mohammad}
}
