@article { ISI:000274436100010,
	title = {Enhanced production of gibberellin A(4) (GA(4)) by a mutant of Gibberella fujikuroi in wheat gluten medium},
	journal = {Journal of Industrial Microbiology \& Biotechnology},
	volume = {37},
	number = {3},
	year = {2010},
	month = {MAR},
	pages = {297-306},
	publisher = {SPRINGER HEIDELBERG},
	address = {TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY},
	abstract = {<p>Mutants of Gibberella fujikuroi with different colony characteristics, morphology and pigmentation were generated by exposure to UV radiation. A mutant, Mor-189, was selected based on its short filament length, relatively high gibberellin A(4) (GA(4)) and gibberellin A(3) (GA(3)) production, as well as its lack of pigmentation. Production of GA(4) by Mor-189 was studied using different inorganic and organic nitrogen sources, carbon sources and by maintaining the pH of the fermentation medium using calcium carbonate. Analysis of GA(4) and GA(3) was done by reversed-phase high-performance liquid chromatography and LC-MS. The mutants of G. fujikuroi produced more GA(4) when the pH of the medium was maintained above 5. During shake flask studies, the mutant Mor-189 produced 210 mg l(-1) GA(4) in media containing wheat gluten as the nitrogen source and glucose as the carbon source. Fed-batch fermentation in a 14 l agitated fermenter was performed to evaluate the applicability of the mutant Mor-189 for the production of GA(4). In 7-day fed-batch fermentation, 600 mg l(-1) GA(4) were obtained in the culture filtrate. The concentration of GA(4) and GA(3) combined was 713 mg l(-1), of which GA(4) accounted for 84\% of the total gibberellin. These values are substantially higher than those published previously. The present study indicated that, along with maintenance of pH and controlled glucose feeding, the use of wheat gluten as the sole nitrogen source considerably enhances GA(4) production by the mutant Mor-189.</p>},
	keywords = {Fermentation, GA(4), Gibberella fujikuroi, Mutant, Wheat gluten},
	issn = {1367-5435},
	doi = {10.1007/s10295-009-0673-1},
	author = {Lale, Geetanjali and Gadre, Ramchandra V.}
}
@article { ISI:000375900200001,
	title = {Production of bikaverin by a Fusarium fujikuroi mutant in submerged cultures},
	journal = {AMB Express},
	volume = {6},
	year = {2016},
	month = {MAY},
	pages = {Article Number: 34},
	publisher = {BIOMED CENTRAL LTD},
	address = {236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND},
	abstract = {<p>A variety of mutants having different colony characteristics, morphology and soluble pigmentation were generated from Fusarium fujikuroi by exposure to UV radiation. Mutants were selected that formed dry, compact, small colonies with reddish-violet pigment on regeneration agar plates. The production of bikaverin by Mut-4 was examined in shake flasks in media with different nitrogen and carbon sources. The optimal C: N ratio for the maximal bikaverin production by Mut-4 was 150: 1. It produced still higher bikaverin (6.3 g l(-1)) in a medium containing defatted cottonseed meal as nitrogen source, in combination with glucose. Bikaverin produced was extracted, purified and characterized by UV-visible and NMR spectroscopy. Bikaverin production in the present investigation was substantially higher than that reported by earlier investigators in submerged and solid-state fermentations.</p>},
	keywords = {Bikaverin, Fusarium fujikuroi, Gibberella fujikuroi, Pigment production, Submerged cultures},
	issn = {2191-0855},
	doi = {10.1186/s13568-016-0205-0},
	author = {Lale, Geetanjali and Gadre, R. V.}
}
