@article { ISI:000276010000034,
	title = {Cytotoxic activity of 9,10-dihydro-2,5-dimethoxyphenanthrene-1,7-diol from Eulophia nuda against human cancer cells},
	journal = {Journal of Ethnopharmacology},
	volume = {128},
	number = {1},
	year = {2010},
	month = {MAR},
	pages = {251-253},
	publisher = {ELSEVIER IRELAND LTD},
	address = {ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND},
	abstract = {<p>Ethnopharmacological relevance: Eulophia nuda L. (Orchidaceae) is a medicinally important terrestrial orchid used for the treatment of tumours and various health problems by the local healers throughout the Western Ghats region in Maharashtra (India). Aim of the study: To isolate the active molecule from Eulophia nuda and to study its cytotoxic potential against human cancer cells. Materials and methods: The crude methanolic extract of Eulophia nuda tubers was fractionated by stepwise gradient of the solvents chloroform-methanol to isolate the pure compound. Isolated pure compound was assessed for its cytotoxic potential against human breast cancer cell lines, MCF-7 and MDA-MB-231 using MTT assay. Structure elucidation of the isolated active compound was carried out by extensive spectroscopic analysis including (1)H NMR, (13)C NMR, NOESY, COSY, LC-MS and IR. Results: The isolated active molecule was identified as phenanthrene derivative 9,10-dihydro-2,5-dimethoxyphenanthrene-1,7-diol. This compound showed good antiproliferative activity against human breast cancer cell lines MCF-7 (91\%) and MDA-MB-231 (85\%) at 1000 mu g/ml concentration. Conclusion: 9,10-Dihydro-2,5-dimethoxyphenanthrene-1,7-diol from Eulophia nuda tubers showed good growth suppressive effect against human cancer cell lines MCF-7 and MDA-MB-231 making it a potential biomolecule against human cancer. (C) 2009 Elsevier Ireland Ltd. All rights reserved.</p>},
	keywords = {Cytotoxic activity, Eulophia nuda, Phenanthrene derivatives},
	issn = {0378-8741},
	doi = {10.1016/j.jep.2009.12.031},
	author = {Shriram, Varsha and Kumar, Vinay and Kishor, P. B. Kavi and Suryawanshi, Sharad B. and Upadhyay, Ankur K. and Bhat, Manoj K.}
}
