@article { ISI:000281058900005,
	title = {Nanogold-Loaded sharp-edged carbon bullets as plant-gene carriers},
	journal = {Advanced Functional Materials},
	volume = {20},
	number = {15},
	year = {2010},
	month = {AUG},
	pages = {2416-2423},
	publisher = {WILEY-V C H VERLAG GMBH},
	address = {PO BOX 10 11 61, D-69451 WEINHEIM, GERMANY},
	abstract = {<p>The higher DNA delivery efficiency into plants by gold nanoparticles embedded in sharp carbonaceous carriers is demonstrated. These nanogold-embedded carbon matrices are prepared by heat treatment of biogenic intracellular gold nanoparticles. The DNA-delivery efficiency is tested on a model plant, Nicotiana tabacum, and is further extended to the monocot, Oryza sativa, and a hard dicot tree species, Leucaena leucocephala. These materials reveal good dispersion of the transport material, producing a greater number of GUS foci per unit area. The added advantages of the composite carrier are the lower plasmid and gold requirements. Plant-cell damage with the carbon-supported particles is very minimal and can be, gauged from the increased plant regeneration and transformation efficiency compared with that of the commercial micrometer-sized gold particles. This is ascribed to the sharp edges that the carbon supports possess, which lead to better piercing capabilities with minimum damage.</p>},
	issn = {1616-301X},
	doi = {10.1002/adfm.200901883},
	author = {Vijayakumar, Periyasamy S. and Othalathara U. Abhilash and Khan, Bashir Mohammad and Bhagavatula L. V. Prasad}
}
