@article { ISI:000271494300030,
	title = {Cellulases from penicillium janthinellum mutants: solid-state production and their stability in ionic liquids},
	journal = {Bioresources},
	volume = {4},
	number = {4},
	year = {2009},
	month = {AUG},
	pages = {1670-1681},
	publisher = {NORTH CAROLINA STATE UNIV DEPT WOOD \& PAPER SCI},
	address = {CAMPUS BOX 8005, RALEIGH, NC 27695-8005 USA},
	abstract = {<p>The cellulase production by P. janthinellum mutants on lignocellulosic material such as cellulose or steam exploded bagasse (SEB) in combination with wheat bran was studied in solid state fermentation (SSF). One of the mutants, EU2D21, produced the highest levels of endoglucanase (3710 IU g(-1) carbon source) and beta-glucosidase (155 IU g(-1) carbon source). Ionic liquids are so-called green solvents that have become attractive for biocatalysis. Stability of mutant cellulases was tested in 10-50\% of the ionic liquid 1-butyl-3-methylimidazolium chloride ([bmim]Cl). FPA and CMCase were significantly stable in 10\% ionic liquid after 5h. beta-glucosidase showed 85\% of its original activity after 5 h incubation in 30\% ionic liquid and retained 55\% of its activity after 24 h. This enzyme preparation hydrolyzed ionic-liquid-treated SEB completely in 15 h in the presence of 20\% ionic liquid. These studies revealed that there is no need of regenerating cellulose after ionic liquid treatment, since cellulase of mutant strain was found to be significantly stable in the ionic liquid.</p>},
	keywords = {Cellulase stability, Ionic liquid, Mutant cellulase, Solid state fermentation},
	issn = {1930-2126},
	author = {Adsul, Mukund G. and Terwadkar, Asawari P. and Varma, Anjanikumar and Gokhale, Digambar V.}
}
@article { ISI:000291194800029,
	title = {Comparative production of cellulases by mutants of penicillium janthinellum NCIM 1171 and its application in hydrolysis of avicel and cellulose},
	journal = {Bioresource Technology},
	volume = {102},
	number = {11},
	year = {2011},
	month = {JUN},
	pages = {6569-6572},
	publisher = {ELSEVIER SCI LTD},
	address = {THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND},
	abstract = {<p>Mutants of Penicillium janthinellum NCIM 1171 were evaluated for cellulase production using both submerged fermentation (SmF) and solid state fermentation (SSF). Mutant EU2D-21 gave highest yields of cellulases in both SmF and SSF. Hydrolysis of Avicel and cellulose were compared using SmF and SSF derived enzyme preparations obtained from EU2D-21. Surprisingly, the use of SSF derived preparation gave less hydrolysis compared to SmF derived enzymes. This may be due to inactivation of beta-glucosidase at 50 degrees C in SSF derived enzyme preparations. SmF derived enzyme preparations contained both thermostable and thermosensitive beta-glucosidases where as SSF derived enzyme preparations contained predominantly thermosensitive beta-glucosidase. This is the first report on less thermostability of SSF derived p-glucosidase which is the main reason for getting less hydrolysis. (C) 2011 Elsevier Ltd. All rights reserved.</p>},
	keywords = {beta-Glucosidase thermostability, Mutant EU2D-21, Solid state fermentation, Submerged fermentation},
	issn = {0960-8524},
	doi = {10.1016/j.biortech.2011.01.014},
	author = {Singhvi, M. S. and Adsul, Mukund G. and Gokhale, D. V.}
}
