@article { ISI:000280972500010,
	title = {Enzymatic hydrolysis and ethanol production using xyloglucanase and debaromyces hansenii from tamarind kernel powder: galactoxyloglucan predominant hemicellulose},
	journal = {Journal of Biotechnology},
	volume = {148},
	number = {4},
	year = {2010},
	month = {AUG},
	pages = {233-239},
	publisher = {ELSEVIER SCIENCE BV},
	address = {PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS},
	abstract = {<p>The hydrolysis and ethanol production from tamarind kernel powder (TKP), a rich source of galactoxyloglucan (GXG) was investigated for the first time using xyloglucanase and thermotolerant Debaromyces hansenii. The acid hydrolysis of TKP with 2N H(2)SO(4) at 120 degrees C for 30 min yielded an overall saccharification of 94\% based on the total available carbohydrate content and further fermentation at 40 degrees C with thermotolerant D hansenii produced an ethanol yield of 0.35 g/g. A maximum hydrolysis of 55 and 78\% for GXG was obtained in 48 h at 50 degrees C using Thermomonospora xyloglucanase (TXy) and accellerase (TM) 1000, respectively. The synergistic effect of beta-galactosidase and xyloglucanase was demonstrated by the exogenous addition of beta-galactosidase to TXy which improved the overall hydrolysis of GXG by 30\%. The rate of hydrolysis of GXG with TXy and accellerase was increased by 15-20\% in the presence of chemical surfactants (tween 80 and toluene) or protein additive (BSA). The fermentation of enzymatic hydrolysates of GXG by TXy and accellerase with free cells at 40 degrees C produced an ethanol yield of 0.39 and 0.41 g/g whereas with immobilized cells produced 0.45 and 0.43 g/g, respectively, with a theoretical conversion efficiencies of 78-88\%. The immobilized yeast cells were reused six times at 40 degrees C with 100\% fermentation efficiency. (C) 2010 Elsevier B.V. All rights reserved.</p>},
	keywords = {Ethanol, Galactoxyloglucan, Hydrolysis, Tamarind kernel powder, Thermotolerant yeast, Xyloglucanase},
	issn = {0168-1656},
	doi = {10.1016/j.jbiotec.2010.06.004},
	author = {Menon, Vishnu and Prakash, Gyan and Rao, Mala}
}
