@article { ISI:000288661500011, title = {Association between chemical and genetic variation in calophyllum inophyllum, a medicinally important tree of the Western Ghats of India}, journal = {Plant Systematics and Evolution}, volume = {292}, number = {3-4}, year = {2011}, month = {APR}, pages = {257-265}, publisher = {SPRINGER WIEN}, address = {SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA}, abstract = {

The objective of the present work is to study the chemical variation in Calophyllum inophyllum growing along the Western Ghats of India. Contents of dipyranocoumarins (inophyllums) in C. inophyllum were determined to assess whether they could be used as a taxonomic marker for C. inophyllum. This study also aims to establish inter simple sequence repeat (ISSR) markers that can be used to study genetic variation within the species and explore correlation between ISSR and chemical markers. The contents of dipyranocoumarins were estimated in seeds collected from 20 locations. Leaves from plants at the same 20 locations were assayed for ISSR variation. A dendrogram based on Nei{\textquoteright}s genetic distance as well as principal component analysis based on dipyranocoumarins and ISSR variation clustered plants from these 20 locations into three groups that indicated close relationship among ISSR, dipyranocoumarins contents, and geographical position (variation) of locations. Based on this study, two locations of elite plants were identified.

}, keywords = {Calophyllum inophyllum, Dendrogram, dipyranocoumarins, HPLC, ISSR, Principal component analysis, Western Ghats of India}, issn = {0378-2697}, doi = {10.1007/s00606-010-0409-8}, author = {Pawar, Kiran D. and Joshi, Swati P. and Thengane, Shubhada Ratnakar} } @article { ISI:000326319700002, title = {Antimycobacterial labdane diterpenes from leucas stelligera}, journal = {Journal of Natural Products}, volume = {76}, number = {10}, year = {2013}, month = {OCT}, pages = {1836-1841}, publisher = {AMER CHEMICAL SOC}, address = {1155 16TH ST, NW, WASHINGTON, DC 20036 USA}, abstract = {

Phytochemical investigation of the acetone extract of the aerial parts of Leucas stelligera afforded four new compounds (1-4) belonging to the labdane diterpene series as well as two known flavones, velutin (5) and chrysoeriol (6). Structure elucidation of the new compounds was carried out using ID and 2D NMR spectroscopic data and single-crystal X-ray crystallography of compound 1. Compounds 1-4 exhibited selective antimycobacterial activity against Mycobacterium tuberculosis with IC50 values in the range 5.02-9.80 mu g/mL.

}, issn = {0163-3864}, doi = {10.1021/np400002p}, author = {Kulkarni, Roshan R. and Shurpali, Ketaki and Puranik, Vedavati G. and Sarkar, Dhiman and Joshi, Swati P.} } @article { ISI:000350032000042, title = {Advanced cell-based high-throughput screening assays targeting active and latent Mycobacterium tuberculosis}, journal = {Journal of Biotechnology}, volume = {185}, number = {S}, year = {2014}, note = {European Biotechnology Congress, Lecce, ITALY, MAY 15-18, 2014}, month = {SEP}, pages = {S18}, publisher = {ELSEVIER SCIENCE BV}, type = {Meeting Abstract}, address = {PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS}, issn = {0168-1656}, doi = {10.1016/j.jbiotec.2014.07.061}, author = {Shurpali, Ketaki Dilip and Singh, Upasana and Sarkar, Shamim Akhtar Sampa and Mishra, Abhishek and Kulkarni, Roshan and Joshi, Swati P. and Sarkar, Dhiman} } @article { ISI:000333939500005, title = {Antifungal dimeric chalcone derivative kamalachalcone E from Mallotus philippinensis}, journal = {Natural Product Research}, volume = {28}, number = {4}, year = {2014}, month = {FEB}, pages = {245-250}, publisher = {TAYLOR \& FRANCIS LTD}, type = {Article}, address = {4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND}, abstract = {

From the red coloured extract (Kamala) prepared through acetone extraction of the fresh whole uncrushed fruits of Mallotus philippinensis, one new dimeric chalcone (1) along with three known compounds 1-(5,7-dihydroxy-2,2,6-trimethyl-2H-1-benzopyran-8-yl)-3-phenyl-2-propen -1-one (2), rottlerin (3) and 4 {\textquoteleft}-hydroxyrottlerin (4) were isolated. The structure of compound 1 was elucidated by 1D and 2D NMR analyses that included HSQC, HMBC, COSY and ROESY experiments along with the literature comparison. Compounds 1-4 were evaluated for antifungal activity against different human pathogenic yeasts and filamentous fungi. The antiproliferative activity of the compounds was evaluated against Thp-1 cell lines. Compounds 1 and 2 both exhibited IC50 of 8, 4 and 16 mu g/mL against Cryptococcus neoformans PRL518, C. neoformans ATCC32045 and Aspergillus fumigatus, respectively. Compound 4, at 100 mu g/mL, showed 54\% growth inhibition of Thp-1 cell lines.

}, keywords = {4 {\textquoteleft}-hydroxyrottlerin, antifungal, dimeric chalcone, kamalachalcone E, Mallotus philippinensis, rottlerin}, issn = {1478-6419}, doi = {10.1080/14786419.2013.843178}, author = {Kulkarni, Roshan R. and Tupe, Santosh G. and Gample, Suvarna P. and Chandgude, Macchindra G. and Sarkar, Dhiman and Deshpande, Mukund V. and Joshi, Swati P.} } @article { ISI:000359850300008, title = {Antimicrobial activity of plant extracts against sexually transmitted pathogens}, journal = {Natural Product Research}, volume = {29}, number = {16}, year = {2015}, month = {AUG}, pages = {1562-1566}, publisher = {TAYLOR \& FRANCIS LTD}, address = {4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND}, abstract = {

Comprehensive management of sexually transmitted infections (STIs) using vaginal or rectal microbicide-based intervention is one of the strategies for prevention of HIV infection. Herbal products have been used for treating STIs traditionally. Herein, we present in vitro activity of 10 plant extracts and their 34 fractions against three sexually transmitted/reproductive tract pathogens - Neisseria gonorrhoeae, Haemophilus ducreyi and Candida albicans. The plant parts were selected; the extracts/fractions were prepared and screened by disc diffusion method. The minimum inhibitory and minimum cidal concentrations were determined. The qualitative phytochemical analysis of selected extracts/fractions showing activity was performed. Of the extracts/fractions tested, three inhibited C. albicans, ten inhibited N. gonorrhoeae and five inhibited H. ducreyi growth. Our study demonstrated that Terminalia paniculata Roth. extracts/fractions inhibited growth of all three organisms. The ethyl acetate fraction of Syzygium cumini Linn. and Bridelia retusa (L.) Spreng. extracts was found to inhibit N. gonorrhoeae at lowest concentrations.

}, keywords = {anti-STI activity, Candida albicans, fractions, Haemophilus ducreyi, microbicide, Neisseria gonorrhoeae, plant extracts}, issn = {1478-6419}, doi = {10.1080/14786419.2014.983919}, author = {Jadhav, Nutan and Kulkarni, Sangeeta and Mane, Arati and Kulkarni, Roshan and Palshetker, Aparna and Singh, Kamalinder and Joshi, Swati P. and Risbud, Arun and Kulkarni, Smita} } @article {42987, title = {Antimycobacterium activity of coumarins from fruit pulp of aegle marmelos (L.) correa}, journal = {International Journal of Basic and Applied Chemical Sciences}, volume = {5}, year = {2015}, month = {JUL}, pages = {39-44}, abstract = {

Phytochemical investigation of n-butanol fraction of acetone extract of Aegle marmelos fruit has afforded four compounds coumarins marmelosin (1), marmin (2) and xanthotoxol (3) and flavonoid kaempferol 3- O-rhamnoside, afzelin (4). All the isolated compounds were evaluated for their antimycobacterium activity against Mycobacterium tuberculosis H37Ra and Mycobacterium bovis. Compounds 1 and 2 exhibited antimycobacterium activity against M. tuberculosis H37Ra with an IC50 12.46 {\textmu}g/mL and 4.31 {\textmu}g/mL respectively whereas, at 100 {\textmu}g/mL, 62.5\% and 82.4\% growth inhibition of M. bovis was observed respectively. Compounds 1 and 2 were also evaluated against two gram positive bacteria Staphylococcus aureus, Bacillus subtilis and one gram negative bacteria Escherichia coli. Compound 1 at 100 {\textmu}g/mL, showed growth inhibition, 64.6\% and 74.9\% of E. coli and B. subtilis respectively.

}, author = {Chinchansure, Ashish A. and Shamnani, Neelam and Arkile, Manisha A. and Sarkar, Dhiman and Joshi, Swati P.} } @article {43493, title = {Acetone and methanol fruit extracts of terminalia paniculata inhibit HIV-1 infection in vitro}, journal = {Natural Product Research}, volume = {31}, year = {2017}, month = {JAN}, pages = {1468-1471}, abstract = {In this study, we report the in vitro anti-HIV1 activity of acetone and methanol extracts of fruit of Terminalia paniculata. Cytotoxicity tests were conducted on TZM-bl cells and peripheral blood mononuclear cells (PBMC), the CC50 values of both the extracts were 260g/mL. Using TZM-bl cells, the extracts were tested for their ability to inhibit replication of two primary isolates HIV-1 (X4, Subtype D) and HIV-1 (R5, Subtype C). The activity against HIV-1 primary isolate (R5, Subtype C) was confirmed using activated PBMC and by quantification of HIV-1 p24 antigen. Both the extracts showed anti-HIV1 activity in a dose-dependent manner. The EC50 values of the acetone and methanol extracts of T. paniculata were 10.3g/mL. The enzymatic assays were performed to determine the mechanism of action which indicated that the anti-HIV1 activity might be due to inhibition of reverse transcriptase (77.7\% inhibition) and protease (69.9\% inhibition) enzymes. }, doi = {10.1080/14786419.2016.1258561}, author = {Durge, Ankita and Jadaun, Pratiksha and Wadhwani, Ashish and Chinchansure, Ashish A. and Said, Madhukar and Thulasiram, H. V. and Joshi, Swati P. and Kulkarni, Smita S.} }