@article { ISI:000257200200028,
	title = {Imaging the stomatal physiology of somatic embryo-derived peanut leaves by scanning electrochemical microscopy},
	journal = {Analytical and Bioanalytical Chemistry},
	volume = {391},
	number = {6},
	year = {2008},
	month = {JUL},
	pages = {2227-2233},
	publisher = {SPRINGER HEIDELBERG},
	type = {Article},
	address = {TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY},
	abstract = {<p>The stomatal physiology, chlorophyll distribution and photosynthetic activity of somatic embryo (SE)- and seedling-derived peanut plants grown in vitro (test tube-grown) and extra vitrum (soil-grown) are investigated using scanning electrochemical microscopy (SECM). This SECM imaging is performed in two different feedback modes, corresponding to oxygen evolution and chlorophyll distribution. More specifically, the oxygen evolution profiles of the in vitro leaves indicate important differences in leaf anatomy between the SE- and seedling-derived leaves. On the other hand, the chlorophyll distribution images show individual stomata of size ca. 27 +/- 5 mu m. Further studies on senescing (aged) leaves reveal interesting voltammograms that vary widely over the stomatal complexes and the surrounding tissues, probably due to the release of electroactive metabolites during chlorophyll breakdown when the leaves turn yellow. Thus, the present investigation could open up new opportunities for characterizing botanical systems using electroanalytical techniques. In addition, it could provide further insights into various areas of current relevance, including signal transduction, cell fate/differentiation and developmental biology.</p>},
	keywords = {leaf senescence, oxygen evolution, photosynthesis, SECM, somatic embryogenesis, stomata},
	issn = {1618-2642},
	doi = {10.1007/s00216-008-2132-7},
	author = {Parthasarathy, Meera and Singh, Shweta and Hazra, Sulekha and Pillai, Vijayamohanan K.}
}
@article { ISI:000271982000020,
	title = {Imaging hydrogen oxidation activity of catalyst-coated perfluoro sulfonic acid-polymer electrolyte membranes using scanning electrochemical microscopy},
	journal = {Journal of Chemical Sciences},
	volume = {121},
	number = {5},
	year = {2009},
	month = {SEP},
	pages = {719-725},
	publisher = {INDIAN ACAD SCIENCES},
	address = {C V RAMAN AVENUE, SADASHIVANAGAR, P B \#8005, BANGALORE 560 080, INDIA},
	abstract = {<p>Scanning Electrochemical Microscopy (SECM) is a unique technique for studying fast heterogeneous kinetics and to map reactivity gradients along the surface of an electrocatalyst, especially when it involves multiple surface sites of varying reactivity. It combines the dual advantages offered by ultramicroelectrode (UME) voltammetry in terms of reduced ohmic drop and insignificant double layer charging contribution with the advantages of imaging by rastering the UME across an electro-active surface. In this work, we demonstrate these distinctive features of SECM in evaluating reactivity gradients on catalyst (Pt/C) coated NafionA (R) films towards hydrogen oxidation activity, a reaction of immense technological relevance. Imaging has been performed in the feedback mode by allowing H(2) evolution at the tip (25 A mu m Pt UME), which is reoxidized at the substrate electrode containing Pt/C-Nafion film. Interesting distribution in H(2) oxidation activity has been observed as a function of potential applied to the Pt/C-Nafion film. In addition, a plot of normalized tip current versus the substrate electrode potential indicates the effect of potential-induced reactivity change in the catalyst-coated membranes. The results of the present investigation are believed to be useful to H(2)/O(2) PEM fuel cells with respect to evaluating reactivity gradients of catalyst-coated polymer electrolyte membranes, which is important to rectify problems related to catalyst utilization.</p>},
	keywords = {hydrogen oxidation, Imaging, Nafion, platinum, SECM},
	issn = {0253-4134},
	doi = {10.1007/s12039-009-0086-8},
	author = {Parthasarathy, Meera and Pillai, Vijayamohanan K.}
}
