@article { ISI:000291804900001,
	title = {Isolation, identification and optimization of a new extracellular lipase producing strain of rhizopus sp.},
	journal = {Applied Biochemistry and Biotechnology},
	volume = {164},
	number = {7},
	year = {2011},
	month = {AUG},
	pages = {969-978},
	publisher = {HUMANA PRESS INC},
	address = {999 RIVERVIEW DRIVE SUITE 208, TOTOWA, NJ 07512 USA},
	abstract = {<p>A lipolytic mesophilic fungus which produces lipase extracellularly was isolated from soil. Based on ITS1-5.8S-ITS4 region sequences of ribosomal RNA, it was concluded that the isolate JK-1 belongs to genus Rhizopus and clades with Rhizopus oryzae. The present paper reports the screening, isolation, identification, and optimization of fermentation conditions for the production of lipase (EC 3.1.1.3). Culture conditions were optimized, and the highest lipase production was observed in basal medium with corn steep liquor as nitrogen source and glucose as carbon source. Maximum lipase production was observed at 72 h, which is about 870 U/ml. Optimization of fermentation conditions resulted in 16-fold enhancement in enzyme production.</p>},
	keywords = {Corn steep liquor, Fermentation, ITS region, Lipase, Medium optimization, Rhizopus},
	issn = {0273-2289},
	doi = {10.1007/s12010-011-9188-0},
	author = {Kantak, Jayshree B. and Bagade, Aditi V. and Mahajan, Siddharth A. and Pawar, Shrikant P. and Shouche, Yogesh S. and Prabhune, Asmita}
}
@article { ISI:000314692400006,
	title = {Evaluation of ethanol production by a new isolate of yeast during fermentation in synthetic medium and sugarcane bagasse hemicellulosic hydrolysate},
	journal = {Annals of Microbiology},
	volume = {63},
	number = {1},
	year = {2013},
	month = {MAR},
	pages = {63-70},
	publisher = {SPRINGER},
	address = {233 SPRING ST, NEW YORK, NY 10013 USA},
	abstract = {<p>A new xylose fermenting yeast was isolated from over-ripe banana by enrichment in xylose-containing medium. The phylogenetic analysis of ITS1-5.8S-ITS2 region sequences of ribosomal RNA of isolate BY2 revealed that it shows affiliation to genus Pichia and clades with Pichia caribbica. In batch fermentation, Pichia strain BY2 fermented xylose, producing 15 g l(-1) ethanol from 30 g l(-1) xylose under shaking conditions at 28A degrees C, with ethanol yield of 0.5 g g(-1) and volumetric productivity of 0.31 g l(-1) h(-1). The optimum pH range for ethanol production from xylose by Pichia strain BY2 was 5-7. Pichia strain BY2 also produced 6.08 g l(-1) ethanol from 30 g l(-1) arabinose. Pichia strain BY2 can utilize sugarcane bagasse hemicellulose acid hydrolysate for alcohol production, efficiency of fermentation was improved by neutralization, and sequential use of activated charcoal adsorption method. Percent total sugar utilized and ethanol yield for the untreated hydrolysate was 17.14\% w/v and 0.33 g g(-1), respectively, compared with 66.79\% w/v and 0.45 g g(-1), respectively, for treated hemicellulose acid hydrolysate. This new yeast isolate showed ethanol yield of 0.45 g g(-1) and volumetric productivity of 0.33 g l(-1) h(-1) from sugarcane bagasse hemicellulose hydrolysate detoxified by neutralization and activated charcoal treatment, and has potential application in practical process of ethanol production from lignocellulosic hydrolysate.</p>},
	keywords = {D-xylose, Ethanol, Pichia, Sugarcane bagasse hemicellulose},
	issn = {1590-4261},
	doi = {10.1007/s13213-012-0445-4},
	author = {Hande, Aparna and Mahajan, Siddharth A. and Prabhune, Asmita}
}
