@article { ISI:000296009600009,
	title = {Influence of abiotic elicitation on production of dipyranocoumarins in suspension cultures of calophyllum inophyllum L.},
	journal = {Acta Physiologiae Plantarum},
	volume = {33},
	number = {6},
	year = {2011},
	month = {NOV},
	pages = {2149-2158},
	publisher = {SPRINGER HEIDELBERG},
	address = {TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY},
	abstract = {<p>Effects of elicitation with heavy metals such as copper, cadmium, chromium (abiotic elicitation) and supplementation of CaCl(2) on production of dipyranocoumarins (inophyllums) in suspension cultures of leaf and stem callus of Calophyllum inophyllum were studied. The optimum timing for elicitor introduction was found to be the 10th day after initiating the suspension cultures. Cadmium as abiotic elicitor in suspension cultures of stem callus was found best to elicit maximum production of inophyllums A, C, and calophyllolide while cadmium in suspension cultures of leaf callus was found best for eliciting maximum production of inophyllums B and P. Inophyllum D was the only dipyranocoumarin whose highest production was achieved when 1.0 mM chromium was used as abiotic elicitor in suspension cultures of stem callus. Out of the three abiotic elicitors used, none could result biomass growth. Only incorporation of CaCl(2) in suspension cultures resulted biomass growth. A maximum of 35.26-fold biomass growth was achieved when suspension cultures of stem callus were incorporated with 2.0 mM CaCl(2). CaCl(2) was noted to have no positive influence on production of most of the dipyranocoumarins under study.</p>},
	keywords = {Abiotic elicitation, Calophyllum inophyllum, dipyranocoumarins, Heavy metals, Suspension culture},
	issn = {0137-5881},
	doi = {10.1007/s11738-011-0754-4},
	author = {Pawar, Kiran D. and Thengane, Shubhada Ratnakar}
}
