@article { ISI:000261282400004,
	title = {Immobilization of penicillin G acylase on amino functionalized mesoporous silica},
	journal = {Research Journal of Biotechnology},
	volume = {3},
	number = {4},
	year = {2008},
	month = {NOV},
	pages = {18-21},
	publisher = {RESEARCH JOURNAL BIOTECHNOLOGY},
	type = {Article},
	address = {SECTOR A-80, SCHEME NO 54, VIJAY NAGAR, A B ROAD, INDORE, 452 010 MP, INDIA},
	abstract = {<p>Penicillin G acylase was immobilized on Amino functionalized mesoporous silica by adsorption method. In this paper we present the effect of penicillin G acylase immobilization on amino-functionalized mesoporous silica and the effect of silica as host matrix on enzyme kinetics. The loading capacity of immobilized Penicillin G acylase is 34 mg protein per 0.5 g of SBA-15. About 65\% of the protein was adsorbed on mesoporous silica. The stability of penicillin acylase was enhanced after immobilization on mesoporous silica. The adsorbed enzyme is more stable than the soluble form, both to temperature and pH environments and retained 73\% of its activity.</p>},
	keywords = {Amino functionalized mesoporous silica, Immobilization, Penicillin G acylase, pH and temperature stabilities},
	issn = {0973-6263},
	author = {Sridevi, N. and Pallavi, Shah and Asmita, Prabhune}
}
@article { ISI:000311755000014,
	title = {Gellan Gum a novel polysaccharide matrix for immobilization of thermo-tolerant yeast cells with invertase activity: factorial design and rheological studies},
	journal = {Research Journal of Biotechnology},
	volume = {7},
	number = {4},
	year = {2012},
	month = {NOV},
	pages = {81-87},
	publisher = {RESEARCH JOURNAL BIOTECHNOLOGY},
	address = {SECTOR A-80, SCHEME NO 54, VIJAY NAGAR, A B ROAD, INDORE, 452 010 MP, INDIA},
	abstract = {<p>The microbial extracellular polysaccharide gellan gum has been explored for its application in whole cell immobilization. Preliminary evaluation of the physicochemical properties of gellan gum in the presence of monovalent cation tetramethyl ammonium chloride (TMACl) was carried out. Gellan gum immobilized cells demonstrated better rheological properties than gellan gum. Attempts have been made to reveal the evaluation of gellan gum as a matrix to immobilize a thermotolerant yeast Kluyveromyces marxianus NCYC 2675. A 3(2) factorial design was used to study the simultaneous effect of two variables. The effect of the polymer concentration and TMACl concentration on various dependent variables like gelling temperature, mean particle size and enzyme activity of the yeast cells was studied. A statistical model with a significant interaction term was obtained to predict the results. Further, optimized immobilized system was evaluated with significant storage stability at 10 C and enhanced reusability with maximum conversion of \&gt; 90 \% when crosslinked with glutaraldehyde.</p>},
	keywords = {Gellan gum, Glutaraldehyde, Immobilization, invertase, Rheology, yeast cells},
	issn = {0973-6263},
	author = {Sulabha, Karandikar and Asmita, Prabhune}
}
