@article { ISI:000336115000029,
	title = {Lipase mediated separation of triterpene structural isomers, alpha- and beta-amyrin},
	journal = {Tetrahedron Letters},
	volume = {55},
	number = {19},
	year = {2014},
	month = {MAY},
	pages = {3122-3125},
	publisher = {PERGAMON-ELSEVIER SCIENCE LTD},
	address = {THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND},
	abstract = {<p>Pentacyclic triterpenoids alpha- and beta-amyrin possess a wide range of biological and pharmacological activities. High structural similarity between these two structural isomers makes their chromatographic separation an ineffective and tedious choice. In this study, Candida rugosa lipase catalyzed separation protocol for the isolation of individual isomers has been developed. In the presence of vinyl acetate as the acyl donor, Candida rugosa lipase carried out acetylation of beta-amyrin more efficiently as compared to alpha-amyrin leading to a kinetic separation. The conditions of transesterification reaction were optimized systematically, which was utilized to separate alpha- and beta-amyrin from a mixture obtained from the latex of Plumeria obtusa. (C) 2014 Elsevier Ltd. All rights reserved.</p>},
	keywords = {Amyrin, Candida rugosa, Kinetic separation, Lipase, Structural isomer},
	issn = {0040-4039},
	doi = {10.1016/j.tetlet.2014.04.015},
	author = {Haldar, Saikat and Kale, Balaji S. and Jadhav, Dipesh D. and Thulasiram, Hirekodathakallu V.}
}
@article {44556,
	title = {Regioselective and efficient enzymatic synthesis of antimicrobial andrographolide derivatives},
	journal = {Bioorganic \& Medicinal Chemistry Letters},
	volume = {28},
	year = {2018},
	month = {APR },
	pages = {1132-1137},
	abstract = {<p>Labdane diterpene andrographolide (1) is a major constituent of Andrographis paniculata and known to exhibit wide spectrum of biological activities. In this study, regioselective monoesters of (1) have been synthesized by using Amano lipase AK (Pseudomonas fluorescens) as a biocatalyst. Amano lipase AK was able to execute highly efficient esterification of hydroxyl group attached to C-14 carbon of (1) in presence of acyl donors. Among the various synthesized derivatives including two novel compounds such as andrographolide-14-propionate (3) and andrographolide-14-caproate (5) displayed antimicrobial activity against Staphylococcus aureus with low minimal inhibitory concentration (MIC) 4 mu g/mL and 16 mu g/mL respectively. Furthermore, they have shown low hemolysis activity at their respective MIC and increase in the permeability of the bacterial cell membrane as delineated by FITC uptake and SEM imaging studies. (C) 2018 Elsevier Ltd. All rights reserved.</p>},
	keywords = {Acylation, Andrographolide, Antimicrobial activity, biocatalysis, Hemolysis, Lipase, Natural product},
	issn = {0960-894X},
	doi = {10.1016/j.bmcl.2018.01.007},
	author = {Patil, Harshal S. and Jadhav, Dipesh D. and Paul, Ajay and Mulani, Fayaj A. and Karegaonkar, Shrikant J. and Thulasiram, Hirekodathakallu V.}
}
