@article {48104,
	title = {Process development for crystalline xylitol production from corncob biomass by Pichia caribbica},
	journal = {Food and Bioproducts Processing},
	volume = {133},
	year = {2022},
	month = {MAY},
	pages = {45-56},
	type = {Article},
	abstract = {<p>
	Xylitol, a commodity chemical, is widely used in nutraceutical and pharmaceutical formulations. Microbial xylitol production is a promising alternative to mitigate current industrial practice issues and offers an environment-friendly sustainable conversion route. This study demonstrates a bioprocess for xylitol production from corncob using a mesophilic yeast Pichia caribbica. Corncob was hydrolyzed by dilute acid and steam explosion to recover fermentable xylose and used as the feed for xylitol production. Activated carbon treatment (3\% w/v) completely removed the biomass-derived inhibitors furfural and hydroxymethyl furfural from the liquid hydrolysate. The fermenting yeast Pichia caribbica produced 124.1 +/- 0.45 g/L xylitol from the detoxified and concentrated corncob hydrolysate with a high yield of 0.80 +/- 0.02 g/g. The crystallized xylitol with 96.5\% purity demonstrated no harmful effects on the cell line used as a control for the in-vitro toxicity studies. This proof of concept can be applied to help scale up for bio-refinery-based large-scale production of xylitol from corncob biomass.(c) 2022 Institution of Chemical Engineers. Published by Elsevier Ltd. All rights reserved.</p>
},
	keywords = {Biomass, Corncob, Detoxification, Fermentation, Toxicity analysis, xylitol},
	issn = {0960-3085},
	doi = {10.1016/j.fbp.2022.02.006},
	author = {Dasgupta, Diptarka and Sidana, Arushdeep and Sarkar, Bipul and More, Snehal and Ghosh, Debashish and Bhaskar, Thallada and Ray, Anjan}
}
@article {48295,
	title = {Food-grade xylitol production from corncob biomass with acute oral toxicity studies},
	journal = {World Journal of Microbiology \& Biotechnology},
	volume = {39},
	year = {2023},
	month = {APR},
	pages = {102},
	type = {Article},
	abstract = {<p>
	Xylitol, a sugar substitute, is widely used in various food formulations and finds a steady global market. In this study, xylitol crystals were produced from corncob by fermentation (as an alternative to the chemical catalytic process) by a GRAS yeast Pichia caribbica MTCC 5703 and characterized in detail for their purity and presence of any possible contaminant that may adversely affect mammalian cell growth and proliferation. The acute and chronic oral toxicity trials demonstrated no gross pathological changes with average weekly weight gain in female Wistar rats at high xylitol loading (LD50 \&gt; 10,000 mg/kg body weight). The clinical chemistry analysis supported the evidence of no dose-dependent effect by analyzing blood biochemical parameters. The finding suggests the possible application of the crystals (\&gt; 98\% purity) as a food-grade ingredient for commercial manufacture pending human trials.</p>
},
	keywords = {Acute oral toxicity, Corncob, Fermentation, Shelf life analysis, xylitol},
	issn = {0959-3993},
	doi = {10.1007/s11274-023-03542-2},
	author = {Dasgupta, Diptarka and Ahuja, Vishal and Singh, Raghuvir and More, Snehal and Mudliar, Sandeep and Kumar, Madan}
}
