@article {50172,
	title = {Tracking plasmodium knowlesi through fecal DNA for monitoring zoonotic transmission in wild macaques across southeast and south asia},
	journal = {Journal of Infectious Diseases},
	volume = {233},
	year = {2026},
	month = {MAY},
	pages = {e1238-e1243},
	type = {Article},
	abstract = {<p>
	We conducted the noninvasive surveillance of Plasmodium knowlesi in wild macaques using 4752 fecal samples collected across 9 endemic countries. Parasite DNA was detected in 390 samples (8.2\%), with positivity rates ranging from 1.4\% to 18.4\%. This provides the first field-based evidence that P. knowlesi DNA in feces shed by macaques and present under natural conditions can be detected. These findings validate fecal sampling as a practical and scalable tool for tracking zoonotic-malaria. The results support integration into forest-runoff and rural wastewater surveillance systems, offering new opportunities for early detection of pathogens and environmental monitoring at the human-wildlife interface. This study validates noninvasive fecal sampling for tracking Plasmodium knowlesi in wild macaques, providing a scalable tool for early pathogen detection and environmental malaria surveillance.</p>
},
	keywords = {environmental surveillance, fecal surveillance, macaque, Plasmodium knowlesi, zoonosis},
	issn = {0022-1899},
	doi = {10.1093/infdis/jiag128},
	author = {Wannigama, Dhammika Leshan and Amarasiri, Mohan and Phattharapornjaroen, Phatthranit and Hurst, Cameron and Modchang, Charin and Cynthia, Bernadina and Miyanaga, Kazuhiko and Cui, Longzhu and Fernandez, Stefan and Melhem, Nada M. and Luk-in, Sirirat and Singer, Andrew C. and Ragupathi, Naveen Kumar Devanga and Htun, Thin Sandi and Sei, Kazunari and Ngamwongsatit, Natharin and Hui, Ng Sin and Shimotai, Yoshitaka and Ounjai, Puey and Kanthawee, Phitsanuruk and Tacharoenmuang, Ratana and Kauba, Alice and Eang, Chorney and Romone, Lavinia and Dharne, Mahesh and De Araujo, Juliana Calabria and Ndatuwong, Lazarus G. and Werawatte, W. K. C. P. and Chanthasiri, Traychit and Zhao, Jinxin and Mori, Hirotake and Besa, V. John Jefferson and Kurt, Ozgur and Kanjanabuch, Talerngsak and Zahraei-Ramazani, Ali Reza and Higgins, Paul G. and Aoyagi, Tetsuji and Kicic, Anthony and Trowsdale, Sam and Hongsing, Parichart and Yang, Xiaonan and Wang, Yangzhong and Khatib, Aisha and Sano, Daisuke and Shibuya, Kenji and Abe, Shuichi and Hamamoto, Hiroshi}
}
