@article {50090,
	title = {Novel cocrystal of quercetagetin: in vitro and in vivo insights into biopharmaceutical performance},
	journal = {Pharmaceutical Research},
	volume = {43},
	year = {2026},
	month = {MAR},
	pages = {927-939},
	type = {Article},
	abstract = {<p>
	Purpose Quercetagetin (QTGN) is a naturally occurring flavonol predominantly sourced from marigold flowers and possesses notable therapeutic potential, including antidiabetic, anticancer, antioxidant, anti-inflammatory, and antiviral properties. However, poor aqueous solubility and in turn bioavailability restrict therapeutic utility of QTGN. Crystal engineering is one of the approaches proven to be fruitful in resolving the solubility issues of many active pharmaceutical ingredients (APIs). Method In the present work, a cocrystal of QTGN using betaine (BET) as coformer viz. Quercetagetin \&amp; sdot;betaine \&amp; sdot;ethanol (QTGN \&amp; sdot;BET \&amp; sdot;EtOH) was synthesized using the solvent evaporation method. It was further characterized using Fourier Transform Infrared Spectroscopy (FTIR), Differential Scanning Calorimetry (DSC), Thermogravimetric analysis (TGA), Powder X-ray diffraction (PXRD), and single crystal XRD (SCXRD). Result FTIR studies confirmed hydrogen bonding between QTGN and BET. PXRD studies showed formation of new crystalline phase. The prepared cocrystal had stoichiometric ratio of 1:1:1 between QTGN, BET, and ethanol forming cocrystal ethanolate and shared robust hydroxyl \&amp; ctdot;carboxylate supramolecular synthon as confirmed by TGA and SCXRD, respectively. Equilibrium solubility study and in vitro dissolution study showed a significant improvement (p \&lt; 0.0001) in aqueous solubility of QTGN upon its cocrystallization with BET. Furthermore, in vivo pharmacokinetic study revealed a 1.28-fold increase in bioavailability of QTGN when formulated as cocrystal solvate. The prepared cocrystal was found to be stable over a period of six months at 40 degrees C and 75\% RH when analyzed using PXRD studies. Conclusion The current work represents a frontier in pharmaceutical formulation, providing a means to fully harness the therapeutic potential of QTGN using cocrystal approach.</p>
},
	keywords = {betaine, Bioavailability, cocrystal, quercetagetin, USP apparatus IV},
	issn = {0724-8741},
	doi = {10.1007/s11095-026-04019-1},
	author = {Suryawanshi, Smita and Shaligram, Parth and Gonnade, Rajesh G. and Patil, Sharvil}
}
@article {49958,
	title = {Structural characterisation, anticancer properties, and BSA binding of 2,6-dipyrazinylpyridines: Insights from experiment and theory},
	journal = {Journal of Molecular Structure},
	volume = {1351},
	year = {2026},
	month = {FEB },
	pages = {144225},
	type = {Article},
	abstract = {<p>
	The phenyl-(2,6-di-2-pyrazinyl)pyridine derivatives (L1, L2) were synthesized using a one-pot Krohnke-type method, starting from 2-acetylpyrazine and substituted benzaldehydes. Their structures were characterized using a combination of spectroscopic (NMR, HRMS) and single-crystal X-ray diffraction techniques, complemented by density functional theory (DFT). Single-crystal X-ray diffraction reveals that L1 crystallizes in the C2/c space group (T = 296 K) with its supramolecular assembly being stabilized by C-H \&amp; ctdot;N and pi-pi stacking interactions, whereas L2 facilitates C-H \&amp; ctdot;N, N-H \&amp; ctdot;pi bifurcated, and pi-pi* interactions. The bio-interaction properties of L1 were studied using fluorescence spectroscopy with bovine serum albumin (BSA) as a model protein. Fluorescence studies demonstrated L1 induces static quenching of BSA, with a binding constant of 5.15 x 104 mol \&amp; sdot;dm-3. Synchronous and three-dimensional fluorescence spectra further demonstrated that L1 brings forth significant conformational changes in BSA. The compounds were evaluated for cytotoxicity against the HCT-116 human colorectal cancer cell line.</p>
},
	keywords = {2, 6-dipyrazinylpyridines, Bovine serum albumin interaction, Cytotoxicity (HCT-116 cells), Density functional theory, Halogenated derivatives, Single-crystal X-ray diffraction},
	issn = {0022-2860},
	doi = {10.1016/j.molstruc.2025.144225},
	author = {Enumula, Sreenivasulu and Shaikh, Javed and Shaikh, Amin and Sheikh, Kounsar N. and Tambe, Pranav and Lande, Dipali N. and Gejji, Shridhar P. and Shaligram, Parth and Gonnade, Rajesh and Bhadbhade, Mohan and Ahmed, Khursheed}
}
