@article {49556,
	title = {Vat-based 3D-bioprinted scaffolds from photocurable bacterial levan for osteogenesis and immunomodulation},
	journal = {Biomacromolecules},
	volume = {26},
	year = {2025},
	month = {JAN},
	pages = {954-966},
	type = {Article},
	abstract = {<p>
	Emerging techniques of additive manufacturing, such as vat-based three-dimensional (3D) bioprinting, offer novel routes to prepare personalized scaffolds of complex geometries. However, there is a need to develop bioinks suitable for clinical translation. This study explored the potential of bacterial-sourced methacrylate levan (LeMA) as a bioink for the digital light processing (DLP) 3D bioprinting of bone tissue scaffolds. LeMA was successfully synthesized, characterized, and used to fabricate 3D-bioprinted scaffolds with excellent printability and physicochemical properties. In vitro studies demonstrated superior cytocompatibility of 15\% w/v LeMA gels compared to 20\% gels. 15\% LeMA gels supported osteogenic differentiation , as evidenced by alkaline phosphatase activity and mineral deposition by MC3T3 pre-osteoblasts. Importantly, the LeMA hydrogels positively modulated the macrophage phenotype, promoting the expression of the anti-inflammatory marker CD206. These findings suggest that 3D-printed LeMA scaffolds can create a favorable microenvironment for bone regeneration, highlighting their potential for tissue repair and regeneration applications.</p>
},
	issn = {1525-7797},
	doi = {10.1021/acs.biomac.4c01264},
	author = {Waidi, Yusuf Olatunji and Wagh, Vasudev S. and Mishra, Shivangi and Jhunjhunwala, Siddharth and Dastager, Syed G. and Chatterjee, Kaushik}
}
