@article {49435, title = {Green synthesis of silver and gold ultra nanocomposites from silk fibroin and their application for treatment of endodontic infections}, journal = {Emergent Materials}, volume = {7}, year = {2024}, month = {JUN}, pages = {2645{\textendash}2660}, type = {Article}, abstract = {

The present study is designed to investigate the antibacterial efficacy of\ B. mori\ silk fibroin based silver and gold ultra bio-nanocomposites (SF-AgUNC and SF-AuUNC) against\ Enterococcus faecalis\ for endodontic disinfection. The SF-AgUNC and SF-AuUNC based irrigant solution was tested\ in- vitro\ against\ Enterococcus faecalis\ (ATCC: 29212). In this direction, SF-AgUNC and SF-AuUNC synthesized by silk fibroin are very important as they are economically cheap and easy to biosynthesize. The synthesis of SF-AgUNC and SF-AuUNC was accomplished by dissolving 1 mM silver nitrate and 0.5 mM auric chloride respectively in 10 ml of fibroin as precursors. The structural and morphological analysis was executed by UV-Vis Spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopic analysis (FTIR), Field Emission Scanning Electron Microscopy (FESEM), Transmission electron microscopy (TEM), Energy-dispersive X-ray spectroscopy (EDAX) and X-ray photoelectron spectroscopy (XPS). The qualitative antimicrobial activity of SF-AgUNC and SF-AuUNC were performed against inoculum of\ Enterococcus faecalis\ containing 1⨯105\ CFU/ ml. The same solutions were tested on dentine specimens inoculated with bacterial cultures and found remarkable biofilm reduction. The structural and morphological analysis reveals the formation of SF-AgUNC and SF-AuUNC having quasi-spherical morphology. The average particle size for SF-AgUNC and SF-AuUNC was same (~ 8 nm). The XRD study confirms that, both SF-AgUNC and SF-AuUNC were crystalline in nature. The bacterial growth inhibition and significant biofilm reduction was observed on dentine specimens treated with SF-AgUNC, SF-AuUNC and positive control. In antibacterial study, at 30 μg/well concentration of SF-AgUNC and SF-AuUNC, 3.13 {\textpm} 0.22 and 1.13 {\textpm} 0.22 cm of zone of inhibition was noted respectively. Moreover, growth of\ Enterococcus faecalis\ was completely inhibited at 20 μg/ml and 30 μg/ml when treated with SF-AgUNC and SF-AuUNC respectively. Hence, the present study concludes that SF-AgUNC and SF-AuUNC can be used as an effective intracanal medicament for the treatment of dental problems with no cyto toxicity.

}, doi = {https://doi.org/10.1007/s42247-024-00757-3}, author = {Pramod C. and Pawar, Jayant and Mane, Deepali P. and Khadase, Ashok N. and Chaudhari, Aditya R. and Ughade, Supriya P. and Chaudhari, Ravindra D. and Agawane, Sachin B.} }