@article { ISI:000242209000043,
	title = {Poly(urethane methacrylate-co-glycidyl methacrylate)-supported-polypropylene biphasic membrane for lipase immobilization},
	journal = {Journal of Membrane Science},
	volume = {285},
	number = {1-2},
	year = {2006},
	month = {NOV},
	pages = {395-403},
	publisher = {ELSEVIER SCIENCE BV},
	type = {Article},
	address = {PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS},
	abstract = {<p>Polypropylene (PP) was hydrophilized by coating followed by UV curing of a blend of 2-hydroxyethyl methacrylate (HEMA) terminated polyurethane prepolymer and glycidyl methacrylate (GMA). This allows formation of a hydrophobic membrane with increased surface hydrophilicity, biocompatibility and stability. Candida rugosa lipase (CRL) was covalently immobilized on this membrane using 5\% glutaraldehyde as a crosslinking agent for post immobilization stabilization of enzyme on membrane. The membrane obtained was placed in a batch membrane reactor where a model esterification of oleic acid with octanol was studied. Under optimum conditions, the biocatalytic membrane gave a specific activity of 796.27 units/mg and 90.26\% activity yield. Moreover, there was 85. 10\% retention of specific activity. The biocatalytic membrane was observed to retain about 84.23\% of its synthetic activity after six cycles. (c) 2006 Elsevier B.V. All rights reserved.</p>},
	keywords = {Candida rugosa lipase, Enzyme immobilization, membrane bioreactor, urethane methacrylate},
	issn = {0376-7388},
	doi = {10.1016/j.memsci.2006.09.009},
	author = {Pujari, N. S. and Vaidya, Bx and Bagalkote, S. and Ponrathnam, S. and Nene, Sanjay}
}
@article { ISI:000254479300039,
	title = {Immobilization of Candida rugosa lipase on poly(allyl glycidyl ether-co-thylene glycol dimethacrylate) macroporous polymer particles},
	journal = {Bioresource Technology},
	volume = {99},
	number = {9},
	year = {2008},
	month = {JUN},
	pages = {3623-3629},
	publisher = {ELSEVIER SCI LTD},
	type = {Article},
	address = {THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND},
	abstract = {<p>Macroporous polymer particles containing surface epoxy groups were synthesized for immobilization of Candida rugosa lipase (CRL). The effect of incorporation of two different sets of monomers [allyl glycidyl ether (AGE) and glycidyl methacrylate (GMA)] and the effect of crosslinking density on immobilization of lipase were studied. AGE-co-EGDM polymers gave higher binding and expression of lipase than GMA-co-EGDM polymers. Optimization of immobilization parameters was done with respect to immobilization time and enzyme loading. Amongst AGE-co-EGDM polymer series, AGE-150 polymer found to give maximum lipase activity yield and therefore evaluated for temperature, pH and storage stability. Under optimum conditions, AGE-150 polymer gave 78.40\% of activity yield. Immobilized lipase on AGE-150 showed a broader pH, higher temperature and excellent storage stability. (C) 2007 Published by Elsevier Ltd.</p>},
	keywords = {Candida rugosa lipase, Enzyme immobilization, Epoxy-activated support},
	issn = {0960-8524},
	doi = {10.1016/j.biortech.2007.07-035},
	author = {Vaidya, Bhalchandra K. and Ingavle, Ganesh C. and Ponrathnam, S. and Kulkarni, B. D. and Nene, Sanjay N.}
}
