@article {49087,
	title = {Biodegradable and 3D printable lysine functionalized polycaprolactone scaffolds for tissue engineering applications},
	journal = {Biomaterials Advances},
	volume = {159},
	year = {2024},
	month = {MAY},
	pages = {213816},
	type = {Article},
	abstract = {<p>
	Tissue engineering (TE) has sparked interest in creating scaffolds with customizable properties and functional bioactive sites. However, due to limitations in medical practices and manufacturing technologies, it is challenging to replicate complex porous frameworks with appropriate architectures and bioactivity in vitro. To address these challenges, herein, we present a green approach that involves the amino acid (L-lysine) initiated polymerization of epsilon-caprolactone (CL) to produce modified polycaprolactone (PCL) with favorable active sites for TE applications. Further, to better understand the effect of morphology and porosity on cell attachment and proliferation, scaffolds of different geometries with uniform and interconnected pores are designed and fabricated, and their properties are evaluated in comparison with commercial PCL. The scaffold morphology and complex internal micro-architecture are imaged by micro-computed tomography (micro-CT), revealing pore size in the range of similar to 300-900 mu m and porosity ranging from 30 to 70 \%, while based on the geometry of scaffolds the compressive strength varied from 143 +/- 19 to 214 +/- 10 MPa. Additionally, the degradation profiles of fabricated scaffolds are found to be influenced by both the chemical nature and product design, where Lys-PCL-based scaffolds with better porosity and lower crystallinity degraded faster than commercial PCL scaffolds. According to in vitro studies, Lys-PCL scaffolds have produced an environment that is better for cell adhesion and proliferation. Moreover, the scaffold design affects the way cells interact; Lys-PCL with zigzag geometry has demonstrated superior in vitro vitality (\&gt;90 \%) and proliferation in comparison to other designs. This study emphasizes the importance of enhancing bioactivity while meeting morphology and porosity requirements in the design of scaffolds for tissue engineering applications.</p>
},
	keywords = {Additive manufacturing, Amino acid, Micro-computed tomography, Polycaprolactone},
	doi = {10.1016/j.bioadv.2024.213816},
	author = {Naik, Sonali S. and Torris, Arun and Choudhury, Namita R. and Dutta, Naba K. and Nair, Kiran Sukumaran}
}
@article {49925,
	title = {Critical advances in biofabrication and biomaterial strategies in tracheal tissue engineering: A comprehensive overview},
	journal = {Advances in Colloid and Interface Science},
	volume = {345},
	year = {2025},
	month = {NOV},
	pages = {103604},
	type = {Article},
	abstract = {<p>
	The trachea is a vital respiratory organ that connects the larynx to the lungs and performs crucial functions. Various conditions can cause severe and often irreversible damage to individuals trachea of all age groups. Tracheal regeneration remains a major challenge in respiratory medicine, requiring a innovative solutions to address various underlying causes. Existing clinical interventions often have significant limitations and associated complications. Tissue engineering has potential, but its effectiveness has been limited due to challenges such as poor durability and insufficient revascularization. This review aims to provide a comprehensive exploration of the landscape of tracheal regeneration, shedding light on the path towards advancements in addressing extensive tracheal defects. It follows a structured approach, introducing various surgical procedures, along with their associated complications. Subsequently, it delves into the myriad biomaterials investigated in the realm of tracheal tissue engineering, emphasizing the significance of design considerations in scaffold fabrication. The review then navigates through various platforms utilized in tracheal tissue engineering and recent innovative approaches employed in this domain. Additionally, it provides insights into the clinical translation of tissueengineered trachea, highlighting recent advancements and challenges encountered in real-world applications. Finally, it discusses the significant challenges and offers a perspective outlook on the future of tracheal tissue engineering. Addressing current limitations and envisioning novel strategies, the review contributes to the ongoing dialogue and progression in this critical field of regenerative medicine.</p>
},
	keywords = {3D printing, 4D printing, Additive manufacturing, Biomaterials, Cell sheet engineering, Organoid printing, Tracheal tissue engineering},
	issn = {0001-8686},
	doi = {10.1016/j.cis.2025.103604},
	author = {Naik, Sonali S. and Dutta, Naba K. and Nair, Kiran Sukumaran and Choudhury, Namita Roy}
}
