@article {48712,
	title = {Permeable polymersomes from temperature and pH dual stimuli-responsive PVCL-b-PLL block copolymers for enhanced cell internalization and lysosome targeting},
	journal = {Biomaterials Advances},
	volume = {151},
	year = {2023},
	month = {AUG},
	pages = {213454},
	type = {Article},
	abstract = {<p>
	A series of dual stimuli-responsive block copolymers comprising temperature-responsive poly(N-vinyl-caprolactam) (PVCL) and biodegradable pH-responsive poly(L-lysine) (PLL) of varying chain length were syn-thesized by a combination of free radical polymerization and ring opening polymerization. The block copolymers formed micelles and vesicles (polymersomes) in response to temperature and pH, respectively, in aqueous so-lution. The nanoassemblies were characterized by transmission electron microscopy and dynamic light scattering techniques. Encapsulation of both hydrophobic and hydrophilic dyes in the polymersomes was shown. Doxo-rubicin (DOX) was loaded in the polymersomes and its controlled release in response to the two stimuli, inde-pendently and jointly, was studied. The drug was found to be released due to stimuli-induced increased permeability without disassembly of the polymersomes. A significant increase in the cellular uptake of the drug-loaded polymersomes at hyperthermia conditions was demonstrated at 41 degrees C and release of the drug upon localization in lysosomes was observed. Cellular internalization pathway of the polymersomes was investigated by competitive inhibition assay and a combination of endocytic pathways dominated by caveolae-mediated mechanism was found to be operative.</p>
},
	keywords = {block copolymers, Controlled release, drug delivery, endocytosis, Polymersomes, Stimuli-responsive},
	doi = {10.1016/j.bioadv.2023.213454},
	author = {Surapaneni, Sai Geetika and Choudhari, Shakeb N. and Avhad, Shankarrao V. and Ambade, Ashootosh V.}
}
@article {49311,
	title = {Photo and pH dual stimuli-responsive block copolymer micelles with defined incorporation of o-nitrobenzyl units in poly(ε-caprolactone) ε-caprolactone) block for controlled release},
	journal = {European Polymer Journal},
	volume = {220},
	year = {2024},
	month = {NOV},
	pages = {113501},
	type = {Article},
	abstract = {<p>
	A series of dual stimuli-responsive block copolymers with varying content of photocleavable o-nitrobenzyl (ONB) ester group pendent in the hydrophobic poly(epsilon-caprolactone) block and pH-cleavable acetal linkage at the junction with hydrophilic poly(ethylene glycol) block is synthesized. The hydrophobic block is a random copolymer synthesized by ring-opening copolymerization of epsilon-caprolactone and ONB-substituted epsilon-caprolactone containing varying compositions of the two monomers. Kinetics of polymerization shows that ONBfunctionalized monomer has lower reactivity than that of the unsubstituted monomer. The series of block copolymers shows self-assembly into well-defined spherical micelles of average size of 150-200 nm in aqueous solution. Photocleavage of ONB groups is studied by NMR and UV-vis spectroscopy, and its extent is determined. The two stimuli viz. . UV light and pH are used individually as well as simultaneously to study the controlled release of the encapsulated drug Camptothecin and the synergistic effect of the two stimuli is demonstrated. The effect of varying content of ONB groups is observed on drug release profile. MTT assay showed non-cytotoxic nature of the polymer. Cell uptake and photoinduced release of doxorubicin (DOX) from the micelles in MDAMB-231 cells is demonstrated.</p>
},
	keywords = {drug delivery, micelles, O-nitrobenzyl, pH-responsive, Photoresponsive, Poly(epsilon-caprolactone)},
	issn = {0014-3057},
	doi = {10.1016/j.eurpolymj.2024.113501},
	author = {Avhad, V. Shankarrao and Choudhari, Shakeb N. and Ambade, V. Ashootosh}
}
