@article { ISI:000243157600086, title = {Physical manipulation of biological and chemical syntheses for nanoparticle shape and size control}, journal = {Applied Physics Letters}, volume = {89}, number = {26}, year = {2006}, month = {DEC}, pages = {Article No. 263105}, publisher = {AMER INST PHYSICS}, type = {Article}, address = {CIRCULATION \& FULFILLMENT DIV, 2 HUNTINGTON QUADRANGLE, STE 1 N O 1, MELVILLE, NY 11747-4501 USA}, abstract = {

A nanosynthesis scheme is demonstrated which renders excellent control of nanoparticle shape, size, and dispersity in a solution based synthesis process. The scheme, termed as percolative microcavity synthesis, involves the use of a granular medium with percolative microcavities which facilitate nearly similar grain size/shape dependent reaction zones limiting intrinsic growth inhomogeneities, enabling particle size/shape control. The viability of the process is demonstrated for the synthesis of gold nanoparticles by a plant extract based biological method as well as a chemical method. (c) 2006 American Institute of Physics.

}, issn = {0003-6951}, doi = {10.1063/1.2424272}, author = {Ogale, Satishchandra B. and Ahmad, Absar and Pasricha, R. and Dhas, Vivek V. and Syed, A.} } @article { ISI:000243572800014, title = {Purification and characterization of a lectin from endophytic fungus fusarium solani having complex sugar specificity}, journal = {Archives of Biochemistry and Biophysics }, volume = {457}, number = {2}, year = {2007}, month = {JAN}, pages = {243-251}, publisher = {ELSEVIER SCIENCE INC}, type = {Article}, address = {360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA}, abstract = {

A lectin from the mycelial extract of an endophytic strain of Fusarium solani was purified. Its hemagglutinating activity was inhibited by glycoproteins possessing N-linked as well as O-linked glycans. The thermodynamics and kinetics of binding of glycans and glycoproteins to F. solani lectin Was Studied using SUH ace plasmon resonance. The lectin showed high affinity for asialofetuin, asia-lofibrinogen, asialofibrinogen, and thyroglobulin: and comparatively low affinity for mucin, fetuin, fibrinogen, and holotransferrin. Glycoproteins showed glycans with significant contribution from enthalpy and positive entropy, suggesting several fold higher affinity than their corresponding g the involvement of non-polar protein-protein interaction. Moreover, the higher affinity of the glycoproteins was due to their faster association rates and low activation energy. (c) 2006 Elsevier Inc. All rights reserved.

}, keywords = {endophytic fungus, Fusarium solani, Lectin, Purification, SPR, Thermodynamic properties}, issn = {0003-9861}, doi = {10.1016/j.abb.2006.10.019}, author = {Khan, Feroz and Ahmad, Absar and Khan, Mohammad Islam} } @article { ISI:000272302200009, title = {Production, purification, and characterization of taxol and 10-DABIII from a new endophytic fungus gliocladium sp isolated from the Indian yew tree, taxus baccata}, journal = {Journal of Microbiology and Biotechnology}, volume = {19}, number = {11}, year = {2009}, month = {NOV}, pages = {1342-1347}, publisher = {KOREAN SOC MICROBIOLOGY \& BIOTECHNOLOGY}, address = {KOREA SCI TECHNOL CENTER \#507, 635-4 YEOGSAM-DONG, KANGNAM-GU, SEOUL 135-703, SOUTH KOREA}, abstract = {

We have isolated endophytic fungi from the Indian yew tree, Taxus baccata, and then screened for taxol production. Out of the 40 fungal cultures screened, one fungus Gliocladium sp. was found to produce taxol and 10-DABIII (10-deacetyl baccatin III). These compounds were purified by TLC and HPLC and characterized using UV-spectroscopy, ESI-MS, MS/MS, and proton NMR. One liter of Gliocladium sp. culture yielded 10 mu g of taxol and 65 mu g of 10-DABIII. The purified taxol from the fungus showed cytotoxicity towards cancer lines HL-60 (leukemia), A431 (epidermal carcinoma), and MCF-7 (breast cancer).

}, keywords = {10 DAB III, Endophytic fungi, Gliocladium sp., Taxol, Taxus baccata}, issn = {1017-7825}, doi = {10.4014/jmb.0904.04041}, author = {Sreekanth, D. and Syed, A. and Sarkar, S. and Sarkar, Dhiman and Santhakumari, B. and Ahmad, Absar and Khan, Mohammad Islam} } @article { ISI:000312542600011, title = {Phytosynthesis of intracellular and extracellular gold nanoparticles by living peanut plant (Arachis hypogaea L.)}, journal = {Biotechnology and Applied Biochemistry}, volume = {59}, number = {6}, year = {2012}, month = {NOV}, pages = {471-478}, publisher = {WILEY-BLACKWELL}, type = {Article}, address = {111 RIVER ST, HOBOKEN 07030-5774, NJ USA}, abstract = {

Inorganic nanomaterials of different chemical compositions are conventionally synthesized under harsh environments such as extremes of temperature, pressure, and pH. Moreover, these methods are eco-unfriendly and cumbersome, yield bigger particles, and agglomerate because of not being capped by capping agents. In contrast, biological synthesis of inorganic nanomaterials occurs under ambient conditions, namely room temperature, atmospheric pressure, and physiological pH. These methods are reliable, eco-friendly, and cheap. In this paper, we report for the first time the extracellular and intracellular synthesis of gold nanoparticles (GNPs) using living peanut seedlings. The formed GNPs were highly stable in solution and inside the plant tissue. Transmission electron microscopy revealed that extracellular GNPs distributions were in the form of monodispersed nanoparticles. The nanoparticles ranged from 4 to 6 nm in size. The intercellular nanoparticles were of oval shape and size ranged from 5 to 50 nm. Both extracellular and intracellular nanoparticles were further characterized by standard techniques. The formed GNPs inside the plant tissue were estimated by inductively coupled plasma spectrometry. This opens up an exciting possibility of a plant-based nanoparticle synthesis strategy, wherein the nanoparticles may be entrapped in the biomass in the form of a film or produced in the solution, both of which have interesting applications.

}, keywords = {cells, extracellular, living plant, Peanut, root, TEM}, issn = {0885-4513}, doi = {10.1002/bab.1049}, author = {Raju, Dugyala and Mehta, Urmil J. and Ahmad, Absar} } @article { ISI:000324663100044, title = {Phase, size and shape transformation by fungal biotransformation of bulk TiO2}, journal = {Chemical Engineering Journal}, volume = {230}, year = {2013}, month = {AUG}, pages = {367-371}, publisher = {ELSEVIER SCIENCE SA}, address = {PO BOX 564, 1001 LAUSANNE, SWITZERLAND}, abstract = {

Nanosynthesis is an ever expanding frontier in recent years in view of its implications to many future technologies covering diverse fields. Considerable progress has been made realizing high quality synthesis of elemental nanomaterials as well as compounds as chemical, biological and physical routes. The issue of technologically viable large scale synthesis still continues to be a challenge. Here we demonstrate a novel environmentally friendly top down approach to nanosynthesis which exploits the strength and peculiarities of fungus based bioleaching in extracting radicals from compounds and then providing them with a reactive as well as capping environment. Thus protein capped nanoparticles of TiO2 (5-28 nm, circular and brookite phase) are formed directly from micron size (150-250 nm, disc shape, anatase phase) powder by exposing them to a medium of fungus Humicola sp. at just 50 degrees C. The fungus Humicola sp. is not only useful for the synthesis of nanoparticles but also for the transformation of shape, phase and size of TiO2. (C) 2013 Elsevier B.V. All rights reserved.

}, keywords = {Anatase, Biotransformation, Brookite, humicola sp, TiO2}, issn = {1385-8947}, doi = {10.1016/j.cej.2013.06.091}, author = {Khan, Shadab Ali and Ahmad, Absar} } @article {46242, title = {Protein-capped metal nanoparticles inhibit tau aggregation in alzheimer{\textquoteright}s disease}, journal = {Acs Omega}, volume = {4}, year = {2019}, month = {JULY}, pages = {12833-12840}, type = {Article}, abstract = {

The Alzheimer{\textquoteright}s disease (AD) therapeutic research is yielding a large number of potent molecules. The nanoparticle-based therapeutics against the protein aggregation in AD is also taking a lead especially with amyloid-beta as a primary target. In this work, we have screened for the first time protein-capped (PC) metal nanoparticles for their potency in inhibiting Tau aggregation in vitro. We present a novel function of PC-Fe3O4 and PC-CdS nanoparticles as potent Tau aggregation inhibitors by fluorescence spectrometry, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and electron microscopy. We demonstrate that the biologically synthesized PC-metal nanoparticles, especially iron oxide do not affect the viability of neuroblastoma cells. Moreover, PC-CdS nanoparticles show dual properties of inhibition and disaggregation of Tau. Thus, the nanoparticles can take a lead as potent Tau aggregation inhibitors and can be modified for specific drug delivery due to their very small size. The current work presents unprecedented strategy to design anti-Tau aggregation drugs, which provides interesting insights to understand the role of biological nanostructures in Alzheimer{\textquoteright}s disease.

}, doi = {10.1021/acsomega.9b01411}, author = {Sonawane, Shweta Kishor and Ahmad, Absar and Chinnathambi, Subashchandrabose} }