@article { ISI:000230506000008, title = {Thidiazuron-induced morphogenesis in tamarind seedlings}, journal = {In Vitro Cellular \& Developmental Biology-Plant}, volume = {41}, number = {3}, year = {2005}, month = {MAY}, pages = {240-243}, publisher = {SPRINGER}, type = {Article}, address = {233 SPRING ST, NEW YORK, NY 10013 USA}, abstract = {

Germination of tamarind seeds in medium containing thidiazuron (TDZ) resulted in induction of nodular protrusions in and around the cotyledonary node meristem. The structures developed radially in well-defined circles and subsequently spread towards the cotyledonary bridge and also in the proximal part of the hypocotyl. The structures developed into shoots on transfer to medium devoid of growth regulators. Histological studies revealed that the protrusions initiated from the nodal meristem and extended to the non-meristematic region between the two meristems and also in the proximal part of the hypocotyl in seedlings germinated in 9.08 mu M TDZ. Newly formed cell layers and less-differentiated meristematic protrusions were also seen. With the increase in the distance from the meristem, the buds were less differentiated; in the proximal part of the hypocotyl only the multiple layers of meristematic cells were noted. With extension of the period of incubation, the TDZ-induced meristematic activity extended laterally in circles towards the neighboring region. The radial spread of the meristematic activity from the center of the nodal meristem was also evident at 18.16 mu M TDZ. From the pattern of the morphogenic development and the histological studies it may be hypothesized that in tamarind, TDZ influences the existing meristems specifically. Subsequently de novo organogenesis is triggered in the neighboring cells.

}, keywords = {Caulogenesis, Histology, morphogenesis, protrusions, thidiazuron}, issn = {1054-5476}, doi = {10.1079/IVP2004611}, author = {Mehta, Urmil J. and Sahasrabudhe, N. and Hazra, Sulekha} } @article { ISI:000257172700016, title = {Accumulation of cadmium in growing peanut (Arachis hypogaea L.) seedlings - its effect on lipid peroxidation and on the antioxidative enzymes catalase and gualacol peroxidase}, journal = {Journal of Plant Nutrition and Soil Science}, volume = {171}, number = {3}, year = {2008}, month = {JUN}, pages = {440-447}, publisher = {WILEY-BLACKWELL}, type = {Article}, address = {COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA}, abstract = {

In plants exposed to high metal concentrations, mechanisms to counteract the oxidative burst are crucial for its survival. To investigate the temporal sequence of physiological reactions of peanut seedlings (Arachis hypogaea L.) to cadmium exposure, seeds were cultured in increasing concentrations of CdCl(2), ranging from 50 to 300 mu M. Germination frequency was scored, and the distributions of Cd in root, stem, and leaves were determined after 2 and 4 weeks of culture. Lipid peroxidation and activities of antioxidative enzymes including catalase (CAT, EC 1.11.1.6) and guaiacol peroxiclase (GPX; EC 1.11.1.7) were estimated in these three parts of the plant. Germination of seedlings was not affected, but the growth of seedlings was severely suppressed with increasing concentrations of CdCl(2) and incubation period. Pattern of Cd distribution in the three organs varied with concentration and period of exposure to Cd. Increased lipid peroxidation was detected in all parts of the developing seedlings with increasing metal accumulation. Catalase and guaiacol peroxidase activity varied in the three parts of the seedlings with concentration of Cd and incubation period. Guaiacol peroxidase activity appears to be more active in scavenging the reactive oxygen species in developing peanut seedlings. The results of the present experiment demonstrate the advantages of a tissue-culture model system in studying the complex network of interactions of various factors in stress tolerance.

}, keywords = {Abiotic stress, heavy metal, stress tolerance, TBARS, thiobarbituric acid, Tissue culture}, issn = {1436-8730}, doi = {10.1002/jpln.200700083}, author = {Kumar, Sunil and Mehta, Urmil J. and Hazra, Sulekha} } @article { ISI:000254736700028, title = {Differential accumulation of manganese in three mature tree species (Holoptelia, Cassia, Neem) growing on a mine dump}, journal = {Current Science}, volume = {94}, number = {5}, year = {2008}, month = {MAR}, pages = {639-643}, publisher = {INDIAN ACAD SCIENCES}, type = {Article}, address = {C V RAMAN AVENUE, SADASHIVANAGAR, P B \#8005, BANGALORE 560 080, INDIA}, abstract = {

Three trees, including Cassia siamea (Cassia), Azadirachta indica (Neem), Holoptelia integrifolia (Holoptelia) belonging to three different families were identified from a manganese mine tailing dump. Manganese content in dump soil and in the stem, green leaves and dry, fallen leaves of the plants was determined. Values were compared with similar samples collected from normal vegetation. Under control condition, manganese content was highest in Cassia. Distribution of metal in samples collected from the dump site revealed that Holoptelia has a special ability to accumulate high amounts of manganese under stress condition followed by Cassia and Neem. There is no literature on metal accumulation in Holoptelia. Mechanism of manganese sequestration in Holoptelia is different from the other two trees growing in the same soil.

}, keywords = {Azadirachta indica, Cassia siamea, Holoptelia integrifolia, hyperaccumulator, manganese, mine dump}, issn = {0011-3891}, author = {Raju, D. and Kumar, Sunil and Mehta, Urmil J. and Hazra, Sulekha} } @article { ISI:000288393700001, title = {Synthesis of gold nanoparticles by various leaf fractions of Semecarpus anacardium L. tree}, journal = {Trees-Structure and Function}, volume = {25}, number = {2}, year = {2011}, month = {APR}, pages = {145-151}, publisher = {SPRINGER HEIDELBERG}, address = {TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY}, abstract = {

Gold nanoparticles (NPs) were synthesized using Semecarpus anacardium leaf extracts in water and the green biomass. Extract prepared at ambient condition by crushing the leaves in deionized water is identified as {\textquoteleft}green extract{\textquoteright}, and that by boiling the leaf pieces as {\textquoteleft}boiled extract{\textquoteright}. The mass remaining after separating the {\textquoteleft}green extract{\textquoteright} is identified as {\textquoteleft}green biomass{\textquoteright}. These components triggered rapid reduction of Au(III) to Au (0) in HAuCl4 solution indicating the natural ability of the leaves of S. anacardium to synthesize NPs in ambient conditions. Green extract produced more NPs compared to the boiled extract suggesting denaturization of some of the useful factors due to boiling. NPs were quantified using UV and ICP-AES analysis. These were characterized using Transmission electron microscopy, Fourier transform infrared spectroscopy and X-ray diffraction. TEM images of the particles formed with green extract, boiled extract and green biomass showed that the particles were of different shapes and sizes.

}, keywords = {Biosynthesis, gold nanoparticles, Green extract, Semecarpus, TEM}, issn = {0931-1890}, doi = {10.1007/s00468-010-0493-y}, author = {Raju, D. and Mehta, Urmil J. and Hazra, Sulekha} } @article { ISI:000312542600011, title = {Phytosynthesis of intracellular and extracellular gold nanoparticles by living peanut plant (Arachis hypogaea L.)}, journal = {Biotechnology and Applied Biochemistry}, volume = {59}, number = {6}, year = {2012}, month = {NOV}, pages = {471-478}, publisher = {WILEY-BLACKWELL}, type = {Article}, address = {111 RIVER ST, HOBOKEN 07030-5774, NJ USA}, abstract = {

Inorganic nanomaterials of different chemical compositions are conventionally synthesized under harsh environments such as extremes of temperature, pressure, and pH. Moreover, these methods are eco-unfriendly and cumbersome, yield bigger particles, and agglomerate because of not being capped by capping agents. In contrast, biological synthesis of inorganic nanomaterials occurs under ambient conditions, namely room temperature, atmospheric pressure, and physiological pH. These methods are reliable, eco-friendly, and cheap. In this paper, we report for the first time the extracellular and intracellular synthesis of gold nanoparticles (GNPs) using living peanut seedlings. The formed GNPs were highly stable in solution and inside the plant tissue. Transmission electron microscopy revealed that extracellular GNPs distributions were in the form of monodispersed nanoparticles. The nanoparticles ranged from 4 to 6 nm in size. The intercellular nanoparticles were of oval shape and size ranged from 5 to 50 nm. Both extracellular and intracellular nanoparticles were further characterized by standard techniques. The formed GNPs inside the plant tissue were estimated by inductively coupled plasma spectrometry. This opens up an exciting possibility of a plant-based nanoparticle synthesis strategy, wherein the nanoparticles may be entrapped in the biomass in the form of a film or produced in the solution, both of which have interesting applications.

}, keywords = {cells, extracellular, living plant, Peanut, root, TEM}, issn = {0885-4513}, doi = {10.1002/bab.1049}, author = {Raju, Dugyala and Mehta, Urmil J. and Ahmad, Absar} } @article { ISI:000317836000016, title = {Extra- and intracellular gold nanoparticles synthesis using live peanut callus cells}, journal = {Current Nanoscience}, volume = {9}, number = {1}, year = {2013}, month = {FEB}, pages = {107-112}, publisher = {BENTHAM SCIENCE PUBL LTD}, address = {EXECUTIVE STE Y-2, PO BOX 7917, SAIF ZONE, 1200 BR SHARJAH, U ARAB EMIRATES}, abstract = {

An important challenge of considerable topical significance in nanotechnology is the development of eco-friendly experimental processes for the synthesis of nanomaterials in large quantities with variable sizes, shapes and chemical compositions. Green synthesis routes for the production of inorganic metal nanoparticles using whole cell of microorganisms and plant extracts are gaining tremendous popularity as these are non-toxic, cheap and occur at ambient conditions. The present work emphasizes on gold nanoparticles synthesis protocol using live plant callus cells. Peanut callus cells when incubated with HAuCl4 solution in ambient conditions reduced the precursor and lead to formation of well dispersed, water soluble extracellular and intracellular gold nanoparticles within 24 hours. The biosynthesis of gold nanoparticles was monitored by UV-visible spectroscopy (UV-Vis) and further characterized by X-ray diffraction analysis (XRD), Energy Dispersive Spectroscopy (EDS) and Selected Area Electron Diffraction (SAED). The particle size distribution shows that the average particle size is 50 nm for extra-and 31 nm for intracellular gold nanoparticles. The nanoparticles may be stabilized by proteins secreted by callus cells. The reduction process is believed to occur enzymatically, thus creating the possibility of a rational, plant cell-based method for the synthesis of nanoparticles over a wide range of chemical compositions.

}, keywords = {Biosynthesis, callus, gold nanoparticles, live cells, Peanut, TEM}, issn = {1573-4137}, author = {Raju, D. and Mehta, Urmil J. and Ahmad, Absar} } @article { ISI:000320148900002, title = {Phytosynthesis of silver nanoparticles by semecarpus anacardium L. leaf extract}, journal = {Materials Letters}, volume = {102}, year = {2013}, month = {JUL}, pages = {5-7}, publisher = {ELSEVIER SCIENCE BV}, address = {PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS}, abstract = {

Green synthesis of silver nanoparticles (AgNPs) using Semecmpus anacardium L leaf extract was studied. The reduction of silver (Ag+) ions was characterized by using UV-vis spectrophotometer showing formation of AgNPs within 15-20 mm. A time dependent reaction showed the increase in the nanoparticles (NPs) with time. Transmission electron microscopy (TEM) analysis showed that the synthesized AgNPs varied from 10 to 25 nm and has spherical shape. The Fourier transform infrared (FTIR) analysis showed that phenols and protein were responsible for the formation of the AgNPs. The energy dispersive spectroscopy (EDAX) analysis confirms the formed NPs were of silver. The quantification of AgNPs was studied by inductive coupled plasma spectrometry (ICP-AES). The important outcome of this work can be value addition to the medicinal plants in synthesis of NPs for biomedical applications. (C) 2013 Elsevier B.V. All rights reserved.

}, keywords = {FTIR, Leaf extract, Nanoparticles, Semecarpus anacardium, synthesis, TEM}, issn = {0167-577X}, doi = {10.1016/j.matlet.2013.03.091}, author = {Raju, D. and Hazra, Sulekha and Mehta, Urmil J.} } @article { ISI:000325763700047, title = {Synthesis of Ag-glyconanoparticles using C-glycosides, their lectin binding studies and antibacterial activity}, journal = {New Journal of Chemistry}, volume = {37}, number = {11}, year = {2013}, month = {AUG}, pages = {3716-3720}, publisher = {ROYAL SOC CHEMISTRY}, address = {THOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLAND}, abstract = {

Two 12-C-glycosyl dodecanoic acids, namely, 1-(alpha-D-mannopyranosyl)-12-dodecanoic acid and 1-(alpha-D-glucopyranosyl)-12-dodecanoic acid were synthesized. Their ability to act as reducing and capping agents for the synthesis of water re-dispersible silver nanoparticles is displayed. These Ag C-glycosyl nanoparticles were later utilized to investigate the carbohydrate-lectin interactions. Furthermore, the specificity of mannoside binding to the surface of the Gram negative bacterium Escherichia coli has been utilized to demonstrate the enhanced antibacterial activity of Ag-C-mannosyl nanoparticles towards this bacterium as compared to Ag-C-glycosyl nanoparticles.

}, issn = {1144-0546}, doi = {10.1039/c3nj00496a}, author = {Ramtenki, Vilas and Raju, D. and Mehta, Urmil J. and Ramana, C. V. and Bhagavatula L. V. Prasad} } @article { ISI:000338818800044, title = {Biological synthesis of cationic gold nanoparticles and binding of plasmid DNA}, journal = {Materials Letters}, volume = {129}, year = {2014}, month = {AUG}, pages = {159-161}, publisher = {ELSEVIER SCIENCE BV}, type = {Article}, address = {PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS}, abstract = {

Nanobiotechnology is the development of eco-friendly experimental processes for the synthesis of nanomaterials. The present work focuses on synthesis of cationic gold nanoparticles (C-GNPs) for biological applications, especially in gene and drug delivery studies. A biosynthesis methodology has been developed for the functionalization of gold nanoparticles to cationic nature. The synthesis of C-GNPs was done by using peanut leaf extract in the presence of cysteamine. The formed C-GNPs were characterized by using UV-visible spectroscopy (UV-vis), the particles sizes and shapes were confirmed by a Transmission electron microscope (TEM) and crystallinity of C-GNPs was characterized by diffraction. The binding of plasmid DNA on the C-GNPs was confirmed by agarose gel electrophoresis. (C) 2014 Elsevier B.V. All rights reserved.

}, keywords = {Biomaterials, Cationic, Nanoparticles, Plasmid DNA, synthesis, TEM}, issn = {0167-577X}, doi = {10.1016/j.matlet.2014.05.021}, author = {Raju, D. and Vishwakarma, Rishi K. and Khan, Bashir Mohammad and Mehta, Urmil J. and Ahmad, Absar} } @article {45977, title = {Extracellular synthesis of silver nanoparticles using living peanut seedling}, journal = {Applied Nanoscience}, volume = {4}, year = {2014}, month = {OCT}, pages = {875-879}, type = {Article}, abstract = {

Synthesis of nanoparticles by environment friendly method is an important aspect of nanotechnology. In the present study, extracellular reduction of silver ions to silver nanoparticles was carried out using living peanut plant. The electron microscopic analysis shows that the formed nanoparticles were of different shapes and sizes. The formed nanoparticles were polydispersed. The shapes of the nanoparticles were spherical, square, triangle, hexagonal and rod. Most of the particles were spherical and 56 nm in size. EDS analysis confirmed the formed nanoparticles were of silver. The crystalline nature of nanoparticles was confirmed by diffraction. This method opens up an exciting possibility of plant-based synthesis of other inorganic nanomaterials. This study confirms the synthesis of extracellular silver nanoparticles by living plant.

}, keywords = {Nanoparticles, Shapes, Silver, synthesis, TEM}, issn = {2190-5509}, doi = {10.1007/s13204-013-0269-y}, author = {Raju, D. and Paneliya, Nikita and Mehta, Urmil J.} } @article { ISI:000336107400002, title = {Living plant-mediated synthesis of different shaped gold nanoparticles}, journal = {Nanoscience and Nanotechnology Letters}, volume = {6}, number = {4}, year = {2014}, month = {APR}, pages = {279-283}, publisher = {AMER SCIENTIFIC PUBLISHERS}, address = {26650 THE OLD RD, STE 208, VALENCIA, CA 91381-0751 USA}, abstract = {

We demonstrate extracellular green synthesis of gold nanoparticles with different shapes and sizes by using live Prosopis juliflora L. seedling. The conversion of ionic to metallic gold nanoparticles by P. juliflora seedling was carried out in short period. The formed nanoparticles were characterized by UV, TEM, Diffraction and EDS. TEM shows well dispersed particles of different shapes and sizes. The percentage of different shapes of nanoparticles was calculated, most of the particles were spherical in shape (75\%). The triangle nanoparticles were 10\%, followed by squares (8\%), hexagonal (5\%) and rods (2\%). The diffraction shows the formed nanoparticles are crystalline in nature and EDS confirms the formed nanoparticles are of gold. This shows P. juliflora seedling can synthesis different shapes of well dispersed nanoparticles.

}, keywords = {Gold, Nanoparticles, Prosopis juliflora, Shapes, synthesis, TEM}, issn = {1941-4900}, doi = {10.1166/nnl.2014.1756}, author = {Raju, D. and Paneliya, Nikita and Mehta, Urmil J.} } @article { ISI:000336464400074, title = {Protein mediated synthesis of Au-Ag bimetallic nanoparticles}, journal = {Materials Letters}, volume = {124}, year = {2014}, month = {JUN}, pages = {271-274}, publisher = {ELSEVIER SCIENCE BV}, address = {PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS}, abstract = {

There is growing need to develop environment friendly method for synthesis of bimetallic nanoparticle that do not use toxic chemicals during their synthesis process. Here, we report synthesis of Au-Ag bimetallic nanoparticles by using macerase enzyme as reducing agent at different temperatures. Bimetallic nanoparticles were synthesized at 80 and 90 degrees C. The intensity of formation of nanoparticles was more at 80 degrees C. The bimetallic nanoparticles are characterized by using UV-vis, TEM and EDAX. The TEM study shows the inner gold and outer silver bimetallic nanoparticles. The formed nanoparticles are 4-20 nm in size and spherical in shape. EDAX confirms the formed bimetallic nanoparticles are of gold and silver. (C) 2014 Elsevier B.V. All rights reserved.

}, keywords = {Enzyme, Gold, Macerase, Particles, Silver, synthesis}, issn = {0167-577X}, doi = {10.1016/j.matlet.2014.03.087}, author = {Raju, D. and Mendapara, Ritul and Mehta, Urmil J.} } @article { ISI:000345054100096, title = {Simple recovery of intracellular gold nanoparticles from peanut seedling roots}, journal = {Journal of Nanoscience and Nanotechnology}, volume = {15}, number = {2}, year = {2015}, month = {FEB}, pages = {1575-1581}, publisher = {AMER SCIENTIFIC PUBLISHERS}, address = {26650 THE OLD RD, STE 208, VALENCIA, CA 91381-0751 USA}, abstract = {

Fabrication of inorganic nanomaterials via a biological route witnesses the formation either extracellularly, intracellulary or both. Whereas extracellular formation of these nanomaterials is cherished owing to their easy and economical extraction and purification processes; the intracellular formation of nanomaterials, due to the lack of a proper recovery protocol has always been dreaded, as the extraction processes used so far were tedious, costly, time consuming and often resulting in very low recovery. The aim of the present study was to overcome the problems related with the extraction and recovery of intracellularly synthesized inorganic nanoparticles, and to devise a method to increasing the output, the shape, size, composition and dispersal of nanoparticles is not altered. Water proved to be much better system as it provided well dispersed, stable gold nanoparticles and higher recovery. This is the first report, where intracellular nanoparticles have been recovered using a very cost-effective and eco-friendly approach.

}, keywords = {gold nanoparticles, Peanut, Recovery, Sonication, Water}, issn = {1533-4880}, doi = {10.1166/jnn.2015.9046}, author = {Raju, D. and Mehta, Urmil J. and Ahmad, Absar} } @article { ISI:000377826000008, title = {Biogenic green synthesis of monodispersed gum kondagogu (Cochlospermum gossypium) iron nanocomposite material and its application in germination and growth of mung bean (Vigna radiata) as a plant model}, journal = {IET Nanobiotechnology}, volume = {10}, number = {3}, year = {2016}, month = {JUN}, pages = {141-146}, publisher = {INST ENGINEERING TECHNOLOGY-IET}, address = {MICHAEL FARADAY HOUSE SIX HILLS WAY STEVENAGE, HERTFORD SG1 2AY, ENGLAND}, abstract = {

An eco-friendly green and one-pot synthesis of highly monodispersed iron (Fe) nanoparticles (NPs) by using a natural biopolymer, gum kondagogu (GK) as reducing and capping agent is proposed. The NPs synthesised were characterised by ultra-violet-visible spectroscopy, transmission electron microscopy, scanning electron microscopy and X-ray diffraction. As the concentration of gum and time increases, the intensity of NPs formation increased. The NPs were highly monodispersed with uniform circular shapes of 2-6 nm in size. The formed NPs were crystalline in nature which was confirmed by diffraction analysis. The conversion ratio of Fe ionic form to NPs was 21\% which was quantified by inductively coupled plasma mass spectroscopy (ICP-MS). Fe is essential for plant growth and development. A study was conducted to examine the effect of these NPs on the growth of mung bean (Vigna radiata). The radical length and biomass was increased in seeds exposed to Fe NPs than the ions. The uptake of Fe NPs by the sprouts was also quantified by ICP-MS, in which Fe was more in mung bean seeds exposed to NPs. The -amylase activity was increased in the seeds exposed to NPs. The observed increase in the biomass by Fe NPs and seed germination may facilitate its application in the agriculture as an important cost-effective method for plant growth.

}, keywords = {alpha-amylase activity, Biogenic green synthesis, biological techniques, Biomass, Cochlospermum gossypium, diffraction analysis, enzymes, Fe, gum concentration, high-monodispersed iron nanoparticles, inductively coupled plasma mass spectroscopy, ionic-to-nanoparticle iron conversion ratio, iron, monodispersed gum kondagogu, monodispersed gum kondagogu iron nanocomposite material, mung bean germination, mung bean growth, Nanobiotechnology, Nanocomposites, nanoparticle formation, Nanoparticles, natural biopolymer, plant growth, plant model, scanning electron microscopy, size 2 nm to 6 nm, transmission electron microscopy, ultraviolet spectra, ultraviolet-visible spectroscopy, Vigna radiata, visible spectra, X-ray diffraction}, issn = {1751-8741}, doi = {10.1049/iet-nbt.2015.0112}, author = {Raju, Dugyala and Mehta, Urmil J. and Beedu, Sashidhar Rao} } @article {43556, title = {Identification of biomolecules involved in the biosynthesis of gold nanoparticles from living peanut seedlings}, journal = {Journal of Nanoscience and Nanotechnology}, volume = {17}, year = {2017}, month = {MAR}, pages = {1787-1795}, abstract = {In present work, we have fabricated gold nanoparticles by treating an aqueous solution of 10(-4) M HAuCl4 with the proteins extracted from roots of in vitro raised peanut seedlings and purified them by FPLC at room temperature. These proteins which tested positive for the reduction of 10(-4) M HAuCl4 were identified as Mannose glucose binding lectin and Ara h 8 allergen isoforms by ESI MS/MS. It is a well known fact that aromatic amino acids possess delocalized pi electrons in their ring structures, rendering them as the best electron donors which can transfer electrons and reduce the acceptor. These results suggest that the aromatic amino acids present in the proteins from the peanut seedlings are the primary biomolecules involved in the reduction of Au (III) to Au (0). We isolated different protein of molecular weight 16.4, 28.3 and 30 kDa involved in the formation of nanoparticles. The protein with molecular weight 16.4 kDa (allergens) yielded NPs of 25-60 nm whereas, the proteins with molecular weight 28.3 kDa and 30 kDa (lectins) yielded highly monodispersed NPs of 5-20 nm in size. The results clearly indicate that the size of nanoparticles can be controlled by different proteins.}, doi = {10.1166/jnn.2017.12879}, author = {Raju, D. and Siddiqui, Ejaz Ahmad and Prasanth, S. and Khan, Bashir M. and Mehta, Urmil J. and Ahmad, Absar} } @article {45749, title = {Optimizing culture conditions for establishment of hairy root culture of semecarpus anacardium L.}, journal = {3 Biotech}, volume = {7}, year = {2017}, month = {APR }, pages = {21}, type = {Article}, abstract = {

Semecarpus anacardium L. is a tree species which produces secondary metabolites of medicinal importance. Roots of the plant have been traditionally used in folk medicines. Different strains of Agrobacterium rhizogenes (A4, ATCC15834 and LBA 9402) were used for induction of hairy roots in in vitro grown tissues of the plant. Hairy root initiation was observed after 25-30 days of infection. Optimum transformation frequency of 61\% was achieved on leaf explants with ATCC15834 strain. Infection time of 30 min resulted in greater transformation frequency compared to 10 and 20 min, respectively. The hairy roots cultured in growth regulator-free semi-solid woody plant medium differentiated into callus. Whole shoots infected with ATCC 15834 were found to produce more transformants upon co-cultivation for 4 (65\%) and 5 (67\%) days. Induction of hairy roots in stem explants infected with ATCC 15834 was lower (52\%) compared to leaves (62\%) after 4 days of co-cultivation. In A4 and LBA9402 strains transformation efficiency was 49 +/- 2.8\% and 36 +/- 5.7\% in shoots after 4 days of co-cultivation. Transformation frequency was higher in ATCC15834 strain, irrespective of explants. The hairy roots of S. anacardium elongated slowly upon transfer to half-strength liquid medium. After 3-4 passages in liquid medium slender hairy roots started differentiating which were separated from the original explants. Visible growth of the roots was observed in hormone-free liquid medium after 2-3 months of culturing. Polymerase chain reaction with gene-specific primers from rol A, B and C genes confirms the positive transformation events.

}, keywords = {Hairy root culture, In vitro culture, rol genes, Semecarpus anacardium, Transformation}, issn = {2190-572X}, doi = {10.1007/s13205-017-0608-x}, author = {Panda, Bhuban Mohan and Mehta, Urmil J. and Hazra, Sulekha} }