@article {47162, title = {Isolation and molecular characterization of thermophilic coprophilous fungus malbranchea cinnamomea GSMBKU from goat dung}, journal = {Journal of Pure and Applied Microbiology}, volume = {13}, year = {2019}, month = {DEC}, pages = {2227-2233}, abstract = {

Thermophilic fungi, found in the varied habitats are valuable sources for potential enzymes and other biochemical metabolites throwing challenges to the mycologists. A study of such fungi will not only enables the biochemists, microbiologists, and the biotechnologists to understand the basic molecular process but also search for novel genes, gene products and reactions. In this study, Malbranchea cinnamomea was isolated from goat dung and characterized based on its morphological and physiological characters, and later confirmed molecularly.

}, doi = {10.22207/JPAM.13.4.36 }, author = {Ajmera, Shanthipriya and Rao, V. Koteswara and Ranipadmini, V. and Merugu, Ramchander and Girisham, Sivadevuni} } @article {47452, title = {Aspergillus derived mycotoxins in food and the environment: prevalence, detection, and toxicity}, journal = {Toxicology Reports }, volume = {8}, year = {2021}, month = {JAN}, pages = {1008-1030}, type = {Article}, abstract = {

Aspergillus\ species are the paramount ubiquitous fungi that contaminate various food substrates and produce biochemicals known as mycotoxins. Aflatoxins (AFTs), ochratoxin A (OTA), patulin (PAT), citrinin (CIT), aflatrem (AT), secalonic acids (SA), cyclopiazonic acid (CPA), terrein (TR), sterigmatocystin (ST) and gliotoxin (GT), and other toxins produced by species of\ Aspergillus\ plays a major role in food and human health. Mycotoxins exhibited wide range of toxicity to the humans and animal models even at nanomolar (nM) concentration. Consumption of detrimental mycotoxins adulterated foodstuffs affects human and animal health even trace amounts. Bioaerosols consisting of spores and hyphal fragments are active elicitors of bronchial irritation and allergy, and challenging to the public health.\ Aspergillus\ is the furthermost predominant environmental contaminant unswervingly defile lives with a 40{\textendash}90 \% mortality risk in patients with conceded immunity. Genomics, proteomics, transcriptomics, and metabolomics approaches useful for mycotoxins{\textquoteright} detection which are expensive. Antibody based detection of toxins chemotypes may result in cross-reactivity and uncertainty. Aptamers (APT) are single stranded DNA (ssDNA/RNA), are specifically binds to the target molecules can be generated by systematic evolution of ligands through exponential enrichment (SELEX). APT are fast, sensitive, simple, in-expensive, and field-deployable rapid point of care (POC) detection of toxins, and a better alternative to antibodies.

}, doi = {10.1016/j.toxrep.2021.04.013}, author = {Navale, Vishwambar and Vamkudoth, Koteswara Rao and Ajmera, Shanthipriya and Dhuri, Vaibhavi} } @article {48427, title = {Pectinase production by aspergillus niger and its applications in fruit juice clarification}, journal = {Journal of Pure and Applied Microbiology}, volume = {16}, year = {2022}, month = {NOV}, pages = {2724-2737}, type = {Article}, abstract = {

Pectinases (EC 3.2.1.15) are a class of enzymes that catalyze the depolymerization or de-esterification reactions that degrade pectic substances. In the present study, we have isolated the\ Aspergillus niger\ strain from soil samples, in India and evaluated pectinase production. The highest pectinase producing\ A. niger\ strain was further evaluated and optimized with various agricultural wastes. Plackett-Burman design (PBD) and Central composite design {\textquoteleft}(CCD){\textquoteright} were used to determine the best parameters for maximum pectinase production. Pectinase activity was increased to 99.21 U/ml after optimizing the production medium using PBD and CCD statistical analysis. A positive correlation of pectinase activity between predicted (112.65 U/ml) and experimental (99.21 U/ml with SD=0.005) optimum was observed. Maximum pectinase was produced by\ A. niger\ under submerged fermentation, utilizing orange peel, which is a cost-effective, adaptable, and environmentally friendly approach. The partially purified pectinase showed significant application for apple juice clarification and showed the ability to degrade pectin and therefore the colour change was observed in apple juice within 120 min. Maximum pectinase was produced by\ A. niger\ using agricultural waste orange peel under submerged fermentation which is an economical, versatile and eco-friendly process and pectinase showed a significant application for apple juice clarification.

}, doi = {10.22207/JPAM.16.4.43}, author = {Wagh, Vrushali and Patel, Hirvitaben and Patel, Nafisa and Vamkudoth, Koteswara Rao and Ajmera, Shanthipriya} }