@article {48121, title = {Crystalline xylitol production from corncob biomass with oral toxicity analysis}, journal = {Industrial Crops and Products}, volume = {187}, year = {2022}, month = {NOV }, pages = {115407}, type = {Article}, abstract = {

Xylitol, a five-carbon sugar alcohol, has a steady global market and finds application as a natural sugar substitute in various food and confectionery products. Biocatalytic xylitol production, although touted as a greener alternative to conventional chemical catalysis, suffers from certain challenges, the primary being high cost of production. This study demonstrates a process for food-grade xylitol production from corncob biomass with energy reduction through two major process modifications. A non-conventional fermentation strategy was adopted whereby adjusting aeration without agitation, xylitol with high yield (0.86 +/- 0.015 g/g), and productivity (0.74 gL(-1)h(-1)) could be produced by a GRAS Pichia caribbica MTCC 5703 strain. Xylitol was recovered from the broth in the form of crystals using a combination of membrane-based filtration and crystallization. The crystals demonstrated similar to 98 \% purity when quantified with H-1 NMR. Oral toxicity analysis of the crystals demonstrated no adverse effect in female Winstar rats (at a loading of 2000 mg/kg body weight of animals). Overall process statistics showed that 0.584 kg of food-grade xylitol crystals could be produced from 3.5 kg of corncob biomass. The two-process modifications during fermentation and xylitol recovery enabled an energy saving of similar to 20.842 kW/kg of crystals, providing tremendous advantages for biorefinery-based large-scale xylitol production from corncob biomass.

}, keywords = {Biomass, Fermentation, Oral toxicity analysis, Wastewater treatment, xylitol}, issn = {0926-6690}, doi = {10.1016/j.indcrop.2022.115407}, author = {Ahuja, Vishal and Dasgupta, Diptarka and Kshirsagar, Siddheshwar and Ghosh, Prasenjit and More, Snehal and Gupta, Piyush and Behera, Babita and Bhaskar, Thallada} } @article {48104, title = {Process development for crystalline xylitol production from corncob biomass by Pichia caribbica}, journal = {Food and Bioproducts Processing}, volume = {133}, year = {2022}, month = {MAY}, pages = {45-56}, type = {Article}, abstract = {

Xylitol, a commodity chemical, is widely used in nutraceutical and pharmaceutical formulations. Microbial xylitol production is a promising alternative to mitigate current industrial practice issues and offers an environment-friendly sustainable conversion route. This study demonstrates a bioprocess for xylitol production from corncob using a mesophilic yeast Pichia caribbica. Corncob was hydrolyzed by dilute acid and steam explosion to recover fermentable xylose and used as the feed for xylitol production. Activated carbon treatment (3\% w/v) completely removed the biomass-derived inhibitors furfural and hydroxymethyl furfural from the liquid hydrolysate. The fermenting yeast Pichia caribbica produced 124.1 +/- 0.45 g/L xylitol from the detoxified and concentrated corncob hydrolysate with a high yield of 0.80 +/- 0.02 g/g. The crystallized xylitol with 96.5\% purity demonstrated no harmful effects on the cell line used as a control for the in-vitro toxicity studies. This proof of concept can be applied to help scale up for bio-refinery-based large-scale production of xylitol from corncob biomass.(c) 2022 Institution of Chemical Engineers. Published by Elsevier Ltd. All rights reserved.

}, keywords = {Biomass, Corncob, Detoxification, Fermentation, Toxicity analysis, xylitol}, issn = {0960-3085}, doi = {10.1016/j.fbp.2022.02.006}, author = {Dasgupta, Diptarka and Sidana, Arushdeep and Sarkar, Bipul and More, Snehal and Ghosh, Debashish and Bhaskar, Thallada and Ray, Anjan} } @article {47762, title = {Process development for detoxification of corncob hydrolysate using activated charcoal for xylitol production}, journal = {Journal of Environmental Chemical Engineering}, volume = {10}, year = {2022}, month = {FEB}, pages = {107097}, type = {Article}, abstract = {The work describes process development for detoxification of corncob hydrolysate using activated carbon for microbial xylitol production. Activated carbon was used as an adsorbent to completely remove the major fermentation inhibitors obtained during dilute acid and steam explosion of biomass. A xylitol yield of 0.78 g/g was achieved from the detoxified hydrolysate in a 5 L fermenter by Pichia caribbica. The spent carbon was effectively regenerated and used for three consecutive cycles of operation. The successive regeneration and reuse of carbon could reduce the operational cost by similar to 38\% and be used as a reference dataset for process scale-up and cost-effective xylitol production.}, keywords = {Activated charcoal, Detoxification, Fermentation, Regeneration, xylitol}, issn = {2213-2929}, doi = {10.1016/j.jece.2021.107097}, author = {Ahuja, Vishal and Kshirsagar, Siddheshwar and Ghosh, Prasenjit and Sarkar, Bipul and Sutar, Ajit and More, Snehal and Dasgupta, Diptarka} } @article {48295, title = {Food-grade xylitol production from corncob biomass with acute oral toxicity studies}, journal = {World Journal of Microbiology \& Biotechnology}, volume = {39}, year = {2023}, month = {APR}, pages = {102}, type = {Article}, abstract = {

Xylitol, a sugar substitute, is widely used in various food formulations and finds a steady global market. In this study, xylitol crystals were produced from corncob by fermentation (as an alternative to the chemical catalytic process) by a GRAS yeast Pichia caribbica MTCC 5703 and characterized in detail for their purity and presence of any possible contaminant that may adversely affect mammalian cell growth and proliferation. The acute and chronic oral toxicity trials demonstrated no gross pathological changes with average weekly weight gain in female Wistar rats at high xylitol loading (LD50 \> 10,000 mg/kg body weight). The clinical chemistry analysis supported the evidence of no dose-dependent effect by analyzing blood biochemical parameters. The finding suggests the possible application of the crystals (\> 98\% purity) as a food-grade ingredient for commercial manufacture pending human trials.

}, keywords = {Acute oral toxicity, Corncob, Fermentation, Shelf life analysis, xylitol}, issn = {0959-3993}, doi = {10.1007/s11274-023-03542-2}, author = {Dasgupta, Diptarka and Ahuja, Vishal and Singh, Raghuvir and More, Snehal and Mudliar, Sandeep and Kumar, Madan} }