@article {44503,
	title = { In vitro propagation of Digitalis lanata Ehrh. through direct shoot regeneration - A source of cardiotonic glycosides},
	journal = {Industrial Crops and Products},
	volume = {121},
	year = {2018},
	month = {OCT},
	pages = {313-319},
	type = {Article},
	abstract = {<p>An efficient method for the in vitro propagation of Digitalis lanata Ehrh. through direct organogenesis from leaf and petiole explants has been standardised. MS basal medium supplemented with cytokinins BAP, KIN, TDZ either alone or along with auxins IAA and NAA at different concentrations were tried. TDZ at 4.54 and 6.81 μmol/l were optimum for direct regeneration of shoots from leaf (4.4 {\textpm} 0.6 shoots/explant), and petiole (3.0 {\textpm} 0.8 shoots/explant) explants respectively. Among the various concentrations of auxins IAA, IBA and NAA tried for rooting, the best response occurred on MS basal medium supplemented with 17.13 μmol/l IAA. On greenhouse transfer about 60\% of the plantlets survived. In vitro raised plantlets were morphologically similar to mother plants. Cardiotonic glycosides digoxin and digitoxin were extracted by modified methods and estimated by HPLC. There were no significant differences in digoxin and digitoxin content in leaves of naturally grown and in vitro raised plants. The method for in vitro propagation of D. lanata through direct organogenesis from leaf and petiole explants reported here will be of great use for the rapid and large scale clonal propagation, production of biomass for extraction of cardiotonic glycosides, ex situ conservation, and improvement through conventional plant breeding and transgenic methods.</p>},
	issn = { 0926-6690},
	doi = {10.1016/j.indcrop.2018.05.019},
	author = {Bhushan Bhusare and John, C. K. and Bhatt, V. P. and Nikam, T. D.}
}
@article {46871,
	title = {Induction of somatic embryogenesis in leaf and root explants of Digitalis lanata Ehrh.: direct and indirect method},
	journal = {South African Journal of Botany},
	volume = {130},
	year = {2020},
	month = {MAY},
	pages = {356-365},
	type = {Article},
	issn = {0254-6299},
	doi = {10.1016/j.sajb.2020.01.012},
	author = {Bhusare, B. P. and John, C. K. and Bhatt, V. P. and Nikam, T. D.}
}
@article {47712,
	title = {Colchicine induces tetraploids in in vitro cultures of digitalis lanata Ehrh.: enhanced production of biomass and cardiac glycosides},
	journal = {Industrial Crops and Products},
	volume = {174},
	year = {2021},
	month = {DEC},
	pages = {114167},
	type = {Article},
	abstract = {This study describes a promising method for tetraploidy induction in the in vitro grown diploid shoots of the cardiotonic herb Digitalis lanata by colchicine treatment to obtain a higher content of the cardiac glycosides{\textquoteright} digoxin and digitoxin. The best frequency of tetraploids (33 \%) induction was observed with 0.5 \% colchicine treatment for 8 h. The content of digoxin and digitoxin in the tetraploids increased by 1.61-fold and 1.73-fold over the control diploids respectively. The higher cardiotonic glycoside production in the tetraploid plants of D. lanata have the potential to satisfy the increasing demand of superior plant material for pharmaceutical industries.},
	keywords = {Cardiac glycosides, Colchicine induced polyploidy, Digitalis lanata, Digitoxin, Digoxin},
	issn = {0926-6690},
	doi = {10.1016/j.indcrop.2021.114167},
	author = {Bhusare, B. P. and John, C. K. and Bhatt, V. P. and Nikam, T. D.}
}
