@article {47346,
	title = {Rapid and accurate nucleobase detection using FnCas9 and its application in COVID-19 diagnosis},
	journal = {Biosensors \& Bioelectronics},
	volume = {183},
	year = {2021},
	month = {JUL },
	pages = {113207},
	type = {Article},
	abstract = {<p>Rapid detection of DNA/RNA pathogenic sequences or variants through point-of-care diagnostics is valuable for accelerated clinical prognosis, as witnessed during the recent COVID-19 outbreak. Traditional methods relying on qPCR or sequencing are tough to implement with limited resources, necessitating the development of accurate and robust alternative strategies. Here, we report FnCas9 Editor Linked Uniform Detection Assay (FELUDA) that utilizes a direct Cas9 based enzymatic readout for detecting nucleobase and nucleotide sequences without transcleavage of reporter molecules. We also demonstrate that FELUDA is 100\% accurate in detecting single nucleotide variants (SNVs), including heterozygous carriers, and present a simple web-tool JATAYU to aid end-users. FELUDA is semi-quantitative, can adapt to multiple signal detection platforms, and deploy for versatile applications such as molecular diagnosis during infectious disease outbreaks like COVID-19. Employing a lateral flow readout, FELUDA shows 100\% sensitivity and 97\% specificity across all ranges of viral loads in clinical samples within 1hr. In combination with RT-RPA and a smartphone application True Outcome Predicted via Strip Evaluation (TOPSE), we present a prototype for FELUDA for CoV-2 detection closer to home.</p>},
	keywords = {CRISPRDx, FELUDA, FnCas9, LFA, SARS-CoV2, SNV detection},
	issn = {0956-5663},
	doi = {10.1016/j.bios.2021.113207},
	author = {Azhar, Mohd and Phutela, Rhythm and Kumar, Manoj and Ansari, Asgar Hussain and Rauthan, Riya and Gulati, Sneha and Sharma, Namrata and Sinha, Dipanjali and Sharma, Saumya and Singh, Sunaina and Acharya, Sundaram and Sarkar, Sajal and Paul, Deepanjan and Kathpalia, Poorti and Aich, Meghali and Sehgal, Paras and Ranjan, Gyan and Bhoyar, Rahul C. and Singhal, Khushboo and Lad, Harsha and Patra, Pradeep Kumar and Makharia, Govind and Chandak, Giriraj Ratan and Pesala, Bala and Chakraborty, Debojyoti and Maiti, Souvik and Indian CoV2 Genomics Genetic Epide}
}
@article {47401,
	title = {Rapid identification and tracking of SARS-CoV-2 variants of concern},
	journal = {Lancet},
	volume = {397},
	year = {2021},
	month = {APR},
	pages = {1346-1347},
	type = {Letter},
	issn = {0140-6736},
	doi = {10.1016/S0140-6736(21)00470-0},
	author = {Chakraborty, Debojyoti and Agrawal, Anurag and Maiti, Souvik}
}
