@article { ISI:000242209000043,
	title = {Poly(urethane methacrylate-co-glycidyl methacrylate)-supported-polypropylene biphasic membrane for lipase immobilization},
	journal = {Journal of Membrane Science},
	volume = {285},
	number = {1-2},
	year = {2006},
	month = {NOV},
	pages = {395-403},
	publisher = {ELSEVIER SCIENCE BV},
	type = {Article},
	address = {PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS},
	abstract = {<p>Polypropylene (PP) was hydrophilized by coating followed by UV curing of a blend of 2-hydroxyethyl methacrylate (HEMA) terminated polyurethane prepolymer and glycidyl methacrylate (GMA). This allows formation of a hydrophobic membrane with increased surface hydrophilicity, biocompatibility and stability. Candida rugosa lipase (CRL) was covalently immobilized on this membrane using 5\% glutaraldehyde as a crosslinking agent for post immobilization stabilization of enzyme on membrane. The membrane obtained was placed in a batch membrane reactor where a model esterification of oleic acid with octanol was studied. Under optimum conditions, the biocatalytic membrane gave a specific activity of 796.27 units/mg and 90.26\% activity yield. Moreover, there was 85. 10\% retention of specific activity. The biocatalytic membrane was observed to retain about 84.23\% of its synthetic activity after six cycles. (c) 2006 Elsevier B.V. All rights reserved.</p>},
	keywords = {Candida rugosa lipase, Enzyme immobilization, membrane bioreactor, urethane methacrylate},
	issn = {0376-7388},
	doi = {10.1016/j.memsci.2006.09.009},
	author = {Pujari, N. S. and Vaidya, Bx and Bagalkote, S. and Ponrathnam, S. and Nene, Sanjay}
}
@article { ISI:000250792200005,
	title = {Immobilization of mushroom polyphenol oxidase on poly(allyl glycidyl ether-co-ethylene glycol dimethacrylate) macroporous beaded copolymers},
	journal = {Reactive \& Functional Polymers},
	volume = {67},
	number = {10},
	year = {2007},
	month = {OCT},
	pages = {905-915},
	publisher = {ELSEVIER SCIENCE BV},
	type = {Article},
	address = {PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS},
	abstract = {<p>Functional, macroporous, beaded copolymers containing epoxy groups were synthesized for immobilization of polyphenol oxidase (PPO) from edible mushroom (Agaricus bisporus). The effect of incorporation of two different sets of monomers such as glycidyl methacrylate (GMA) and allyl glycidyl ether (AGE) and the effect of cross-linking agent ethylene glycol dimethacrylate (EGDM) with varying cross-link densities on binding and expression of mushroom PPO activity were studied. The effect of porogen viz. cyclohexanol and hexanol on PPO immobilization was studied. AGE copolymers with hexanol as a porogen were found to give higher binding and expression of PPO activity than GE polymers. Crosslinking of amino groups of enzyme with 5\% glutaraldehyde for 6 h gave a stable binding of PPO on AGE-75(Hex) polymer with storage half-life of approximately 25 days. Under optimum conditions, AGE-75(Hex) polymer gave 70.3\% of activity yield while percent retention of PPO activity was found to be 83.5\%. Immobilized PPO showed a broader pH, higher temperature and excellent storage stability. (c) 2007 Elsevier Ltd. All rights reserved.</p>},
	keywords = {cross-linking agent, Enzyme immobilization, Epoxy-activated support, mushroom PPO},
	issn = {1381-5148},
	doi = {10.1016/j.reactfunetpolym.2007.05.015},
	author = {Vaidya, Bhalchandra K. and Karale, Abhijeet J. and Suthar, Hitesh K. and Ingavle, Ganesh C. and Pathak, Tara Sankar and Ponrathnam, S. and Nene, Sanjay}
}
