@article {49697,
	title = {Engineered silk matrix as a substitute for acellular dermal matrix in breast reconstruction surgery},
	journal = {International Journal of Biological Macromolecules},
	volume = {312},
	year = {2025},
	month = {JUN},
	pages = {144017},
	type = {Article},
	abstract = {<p>
	Implant-based breast reconstruction is a common standard of care for breast cancer patients following mastectomy. To support implant weight and placement, surgeons utilize autologous tissues, acellular dermal matrices (ADMs), or synthetic meshes. While ADMs provide structural support, they present significant risks, including infection transmission and seroma. Conversely, synthetic meshes exhibit poor cellular adhesion, leading to inadequate tissue integration. To address these challenges, there is a need for a matrix that enhances tissue integration while minimizing infection risks and other complications. Silk fibroin (SF), a natural biopolymer, possesses excellent biocompatibility and mechanical properties. This study introduces a novel engineered silk matrix (ESM) as an advanced solution for soft tissue regeneration, specifically in breast reconstruction surgery. The present study establishes the safety of ESM and evaluates its potential a tissue regeneration matrix through extensive in-vitro and in-vivo analyses. In-vitro assays demonstrated superior cellular adhesion, proliferation of human mammary fibroblast cells (HMFCs), collagen deposition and angiogenesis in ESM compared to collagen matrices and ADMs. Safety assessments, conducted in accordance with ISO 10993 guidelines, confirmed noncytotoxic nature of ESM. Furthermore, subcutaneous implantation revealed no systemic toxicity or adverse tissue reactions. In-vivo studies utilizing a Yorkshire pig model of simulated breast reconstruction surgery, demonstrated superior performance of ESM over collagen matrices in tissue regeneration. The findings showed enhanced fibroblast density, increased collagen deposition, and improved vascularization. These results suggest that ESM is a safer and more effective alternative to ADMs in breast reconstruction, with the potential to revolutionize post-mastectomy care for breast cancer patients.</p>
},
	keywords = {Biocompatibility, Breast reconstruction, ISO 10993, Polymer, Safety, Silk protein, tissue regeneration},
	issn = {0141-8130},
	doi = {10.1016/j.ijbiomac.2025.144017},
	author = {Deshpande, Rucha and Shukla, Swati and Kale, Amod and Sayyad, Raeesa and Kewale, Bhawana and Deshmukh, Narendra and Nisal, Anuya and Venugopalan, Premnath}
}
@article {49764,
	title = {Engineered silk-dressing for acceleratedwound healing: biocompatibility and efficacy studies},
	journal = {Macromolecular Bioscience},
	volume = {25},
	year = {2025},
	month = {NOV},
	pages = {e00323},
	type = {Article},
	abstract = {<p>
	Advanced wound care dressings are essential for improving clinical outcomes. The present study investigates the wound management potential of a unique dressing fabricated from silk proteins. The dressing was characterized for its physical and structural properties, including surface texture, porosity, fluid absorption capacity, and moisture vapor transmission rate. These parameters have been found to be critical for optimal wound healing. In vivo full thickness wound healing studies in a rat model validated the efficacy of the Silk-dressing compared to conventional cotton gauze and commercial polyurethane foam dressings. Histopathological analysis confirmed improved re-epithelialization, collagen deposition, angiogenesis, and formation of secondary follicles. Key advantages of Silk-dressing included non-adherence, absorption of exudate, maintenance of optimal moisture at wound site and acceleratedwound closure. Biocompatibility studies were also conducted in accordance with ISO 10993 guidelines, demonstrating no cytotoxicity, irritation, sensitization, or pyrogenicity. These findings highlight the potential of this uniquely designed Silk-dressing as a superior alternative for wound management, with a potential to improve clinical outcomes.</p>
},
	keywords = {Biocompatibility, biopolymer, ISO 10993, Silk protein, wound Healing},
	issn = {1616-5187},
	doi = {10.1002/mabi.202500323},
	author = {Deshpande, Rucha and Pillai, Lakshmi R. and Sayyad, Raeesa and Shukla, Swati and Nisal, Anuya and Venugopalan, Premnath}
}
