@article {44357,
	title = {Favorable 2 {\textquoteright}-substitution in the loop region of a thrombin-binding DNA aptamer},
	journal = {Bioorganic \& Medicinal Chemistry Letters},
	volume = {28},
	year = {2018},
	month = {JUN},
	pages = {1765-1768},
	type = {Article},
	abstract = {Simple 2{\textquoteright}-OMe-chemical modification in the loop region of the 15mer G-rich DNA sequence GGTTGGTGTGGTTGG is reported. The G-quadruplex structure of this thrombin-binding aptamer (TBA), is stabilized by single modifications (T -> 2{\textquoteright}-OMe-U), depending on the position of the modification. The structural stability also renders significantly increased inhibition of thrombin-induced fibrin polymerization, a process closely associated with blood-clotting. (C) 2018 Elsevier Ltd. All rights reserved.},
	doi = {10.1016/j.bmcl.2018.04.027},
	author = {Awachat, Ragini and Wagh, Atish A. and Aher, Manisha and Fernandes, Moneesha and Kumar, Vaijayanti A.}
}
@article {46999,
	title = {Methoxymethyl threofuranosyl thymidine (4 {\textquoteleft}-MOM-TNA-T) at the T7 position of the thrombin-binding aptamer boosts anticoagulation activity, thermal stability, and nuclease resistance},
	journal = {ACS Omega},
	volume = {5},
	year = {2020},
	month = {JAN },
	pages = {498-506},
	type = {Article},
	abstract = {<p>The synthesis of 4{\textquoteright}-methoxymethyl threofuranosyl (4{\textquoteright}-MOM-TNA) thymidine and derived oligomers of the G-rich thrombin-binding aptameric (TBA) sequence is reported. The G-quadruplex stability, anticoagulation activity, and the enzymatic stability of these oligomers bearing the 2{\textquoteright}-3{\textquoteright}-phosphodiester backbone as single substitutions in the loop regions are studied. Amongst all the oligomers, TBA-7T bearing the 4{\textquoteright}-MOM-TNA unit at the T7 position formed a quadruplex with the highest thermal stability. It also resulted in enhanced anticlotting activity that allowed a one-third reduction in the dose, relative to TBA. Further, TBA-7T exhibited enhanced nuclease resistance properties to both endo- and exonucleases.</p>
},
	issn = {2470-1343},
	doi = {10.1021/acsomega.9b03042},
	author = {Varada, Manojkumar and Aher, Manisha and Erande, Namrata and Kumar, Vaijayanti A. and Fernandes, Moneesha}
}
@article {49010,
	title = {Implications of natural 3'-5'- linkages in the loop region of isomeric 2'-5'-linked thrombin-binding aptamer},
	journal = {Chemistryselect},
	volume = {9},
	year = {2024},
	month = {APR },
	type = {Article},
	abstract = {<p>
	The backbone modification of the thrombin-binding aptamer (TBA) in the TT and TGT loop regions by isomeric 2 {\textquoteleft}-5 {\textquoteleft}-linkages was found to impose additive destabilizing effects on the thermal stability of the G-quadruplex structure. In contrast, the thermal stability of isomeric 2 {\textquoteleft}-5 {\textquoteleft}-linked TBA, i. e., isoTBA, was significantly improved by isomeric 3 {\textquoteleft}-5 {\textquoteleft}-phosphodiester linkages. The isoTBA, when modified with 3 {\textquoteleft}-5 {\textquoteleft}-linkages in both lateral TT loops (isoTBA202), exhibited higher thermal stability and enzymatic stability in comparison to other oligomers in the present study, and TBA202 showed higher antithrombin activity than other loop-modified TBA oligomers.</p>
},
	keywords = {2 {\textquoteleft}-5 {\textquoteleft}-linked-linked DNA, DNA-isoDNA chimera, isoDNA, loop-modification, thrombin-binding aptamer},
	issn = {2365-6549},
	doi = {10.1002/slct.202304254},
	author = {Aher, Manisha and Kumar, Vaijayanti A. and Fernandes, Moneesha}
}
