@article {46756, title = {Understanding fundamentals of hepatocellular carcinoma to design next-generation chitosan nano-formulations: Beyond chemotherapy stride}, journal = {Journal of Drug Delivery Science and Technology}, volume = {58}, year = {2020}, month = {AUG}, pages = {101723}, type = {Article}, abstract = {

Hepatocellular carcinoma (HCC) is the deadliest form of liver cancer. Clinically, the main strategies currently being used for the treatment of HCC are surgery, radiotherapy and chemotherapy. Conventional chemotherapy has major drawbacks such as poor bioavailability, high-dose requirements, adverse side effects, low therapeutic indices, and non-specific drug targeting. Therefore, targeted drug delivery systems are fast becoming new tools for the selective killing of cancer cells. Chitosan (CS) is a biodegradable, biocompatible, cationic and natural biopolymer that also exhibits anti-cancer property which is now being explored as a promising candidate for targeted drug delivery. This review outlines an overview of the causative agents, microenvironment, pathophysiology, surface-biomarkers and physiological barriers of HCC. Then, the cellular internalization pathways of nanomedicine and the important physicochemical properties of delivery agents are discussed. The benefits of targeted therapy over conventional therapy with regard to HCC are also discussed. The main objective of this review was to summarize the current knowledge in the field of chitosan-based drug delivery for the management of HCC along with its limitations in a comprehensive and systematic way. This review attempts to provide a holistic roadmap for designing the next-generation chitosan-based drug delivery systems for HCC management.

}, keywords = {chitosan nanoparticles, Hepatocellular carcinoma, Pathophysiology, Surface biomarkers, Targeted Drug Delivery, Tumor microenvironment}, issn = {1773-2247}, doi = {10.1016/j.jddst.2020.101723}, author = {Jaiswal, Neha and Chaudhari, Ravindra D. and Chaudhari, Bhushan P.} } @article {48917, title = {Understanding critical aspects of liposomal synthesis for designing the next generation targeted drug delivery vehicle}, journal = {Chemistryselect}, volume = {8}, year = {2023}, month = {OCT }, type = {Article}, abstract = {

We identified process parameters of the thin film hydration technique and intrinsic factors to synthesize liposomes for drug delivery. The thin film formation step impacted the nature of the lipid layer, and we optimized 240 RPM rotation speed, 700 mm of Hg vacuum pressure, and 2 ml of chloroform as the organic solvent. The hydration step controlled the particle specifications, and we optimized 270 RPM rotation speed, PBS as the hydrating medium, and 1 h hydration time. We obtained a comparatively smaller liposomal population with a lower size distribution just after hydrating the lipid layer that required milder downsizing steps -10 extrusion passes through a single polycarbonate membrane. The intrinsic factors including the concentrations and molar ratio of lipids affected the synthesis steps and the particle specifications. Characterization of liposomes by analytical techniques confirmed the synthesis of a monodisperse population with hydrodynamic diameter\<150 nm, moderate stability, spherical morphology, and high thermal and storage stability. This comprehensive study defines the role of every parameter, provides a mechanistic insight into synthesis that is supported by experimental data, and helps tune specific parameters to synthesize liposomes for drug delivery or any application with desired specifications.

}, keywords = {carrier system, designing and characterization, drug delivery, liposomes, targeting moiety}, issn = {2365-6549}, doi = {10.1002/slct.202302435}, author = {Moudgil, Aliesha and Chaudhari, Bhushan P.} } @article {49974, title = {Understanding the antimicrobial and antioxidant potential of bioinspired platinum nanoparticles synthesized using ocimum tenuiflorum}, journal = { BioNanoScience}, volume = {15}, year = {2025}, month = {JAN}, pages = {1789}, type = {Article}, abstract = {

The emergence of infectious diseases has highlighted the need for novel and effective antimicrobial agents. Platinum nanoparticles (PtNPs) could act as a potent antimicrobial agent due to their unique physicochemical properties. However, the chemical or physical synthesis methods of PtNPs have drawbacks, like irregular shape and use of toxic chemicals. These drawbacks can be effectively overcome by using the biological method synthesis. Thus, this study presents the green synthesis of platinum nanoparticles (PtNPs) using\ Ocimum tenuiflorum\ leaf extract as a bio-reductant. The study also comprehensively encompasses the role of multiple process parameters for the green synthesis of PtNPs. The high-resolution transmission electron microscopy (HR-TEM) revealed spherical PtNPs with an average size of 2.36 {\textpm} 0.5 nm that exhibits excellent stability (zeta potential, -45.67 {\textpm} 2.57 mV). Phytochemical analysis of aqueous\ O. tenuiflorum\ leaf extract was performed to assess the bioactive compounds like saponins, phenols, and tannins, reducing sugars and flavonoids. The PtNPs demonstrated significant antioxidant activity of 86.9 {\textpm} 0.12\% at 10 {\textmu}g/mL. Further, the biosynthesized PtNPs showed efficient antimicrobial activity with a minimum inhibitory concentration (MIC) of 6.25 {\textmu}g/mL against\ Escherichia coli\ and 1.5 {\textmu}g/mL against\ Staphylococcus aureus. These findings highlight the therapeutic potential of eco-friendly PtNPs for pharmaceutical and biotechnological applications.

}, author = {Varma, Sanjana and Bamb, Aagam Lalit and Tambe, Sayali A. and Burade, Dimpal K. and Jagdale, Swati and Pande, Bharat and Vamkudoth, Koteswara Rao and Chaudhari, Bhushan P.} }