@article {46098,
	title = {Use of Plackett-Burman design for enhanced phytase production by Williopsis saturnus NCIM 3298 for applications in animal feed and ethanol production},
	journal = {3 Biotech},
	volume = {9},
	year = {2019},
	month = {JUN},
	pages = {237},
	type = {Article},
	abstract = {<p>Distiller-dried grain solid (DDGS), a co-product of alcohol production, contains cereal grain residues, proteins, and yeast metabolites, which make it suitable in poultry feeding. However, high phytate content of DDGS limits its applicability in poultry feed. In this study, Plackett-Burman design was used to improve cell-bound phytase production by Williopsis saturnus NCIM 3298, and we achieved an enzyme activity of 269IU/g of dry-wet biomass. The effect of this enhanced phytase-displaying yeast strain on hydrolysis of corn phytate and subsequently on ethanol production and DDGS quality was then investigated. Results of saccharification in the presence of phytase showed that reducing sugar content of liquefied mash increased by 11\%, which subsequently improved the ethanol production by 18\% (w/v) (p\&lt;0.01) compared with the control. Notably, phytase treatment decreased the phytate content of corn by 70\% (p\&lt;0.01) compared with the control, thereby improving the availability of free phosphate in fermentation broth and DDGS. Thus, the results obtained suggest that the addition of W. saturnus NCIM 3298 strain has the potential of providing a new source of phytase that would be useful in the feed and ethanol industries.</p>
},
	keywords = {DDGS, Ethanol, Phytase, Plackett-Burman Design, Williopsis saturnus NCIM 3298},
	issn = {2190-572X},
	doi = {10.1007/s13205-019-1764-y},
	author = {Pable, Anupama A. and Shah, Sarah and Kumar, V. Ravi and Khire, Jayant M.}
}
@article {47778,
	title = {Performance of Aspergillus niger (NCIM 563) phytase based feed supplement for broiler growth and phosphorus excretion},
	journal = {Biocatalysis and Agricultural Biotechnology},
	volume = {31},
	year = {2021},
	month = {JAN},
	pages = {101887},
	type = {Article},
	abstract = {Despite availability of commercial enzymes, the phytase produced from relatively inexpensive systems with high yields are gaining global attention in the feed industries in post-antibiotic era. We studied A. niger NCIM 563 Phytase produced in solid state fermentation (SSF) derived Koji powder and evaluated its utility in the poultry feed for broiler growth performance and phosphorous (P) excretion. The ability of phytase in the dried powder was estimated to dephytinize the poultry feed under simulated gastric conditions. Poultry feed was formulated using A. niger NCIM 563 phytase followed by a 42 days feed trial on broilers. After supplementation of phytase to the diet, there was a reduction of dietary P, maintained growth performance, skeletal development of broilers and reduced levels of phytic acid and available P in the litter. Extracellular phytase was able to replace up to 0.1\% P in poultry feed. Minimal downstream processing a low-cost feed supplement with significant phytase activity could provide added advantage for anti-nutrition free poultry feed.},
	keywords = {Phosphorus, Phytase, Poultry feed, Solid state fermentation},
	isbn = {1878-8181},
	doi = {10.1016/j.bcab.2020.101887},
	url = {https://www.sciencedirect.com/science/article/pii/S1878818120319186},
	author = {Puppala, Kumar Raja and Buddhiwant, Priyanka G. and Agawane, Sachin B. and Kadam, Avinash S. and Mote, Chandrashekhar S. and Lonkar, Vijaysinh D. and Khire, Jayant M. and Dharne, Mahesh S.}
}
