@article {49581,
	title = {Low-cost gelatin/collagen scaffolds for bacterial growth in bioreactors for biotechnology},
	journal = {Applied Microbiology and Biotechnology},
	volume = {109},
	year = {2025},
	month = {MAY},
	pages = {113},
	type = {Article},
	abstract = {<p>
	A wide array of pharmaceutical and industrial products available in today{\textquoteright}s market stems from bioreactors. Meeting the escalating demand for these products necessitates significant enhancements in biotechnological processes. This study focuses on developing cost-effective scaffolds designed explicitly for use within bioreactors, employing commonly used polymers such as gelatin and collagen. Bacterial proliferation assays involving Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa were conducted to assess the effectiveness of these scaffolds. The scaffolds were produced by electrospinning polymeric solutions with varying concentrations of gelatin and collagen and were characterized using scanning electron microscopy, Fourier transform infrared spectroscopy, differential scanning calorimetry, and thermogravimetric analysis. Results revealed that scaffolds with 15\% gelatin increased the 24-h proliferation of S. aureus, P. aeruginosa, and E. coli by 52\%, 35\%, and 20\%, respectively. In the case of E. coli, scaffolds with lower gelatin concentrations (1-10\%) were more effective, leading to 35-55\% proliferation growth. These findings highlight the potential application of gelatin/collagen scaffolds in fabricating industrial products derived from these bacteria.</p>
},
	keywords = {Bacterial cell proliferation, collagen, gelatin, Polymeric electrospinning, scaffolds},
	issn = {0175-7598},
	doi = {10.1007/s00253-025-13491-5},
	author = {Pompa-Monroy, Daniella Alejandra and Vera-Graziano, Ricardo and Dastager, Syed G. and Perez-Gonzalez, Graciela Lizeth and Bogdanchikova, Nina and Iglesias, Ana Leticia and Villarreal-Gomez, Luis Jesus}
}
@article {49956,
	title = {Stimulant immobilized bioactive film of functionalized egg albumin blend for wound healing},
	journal = {International Journal of Pharmaceutics},
	volume = {682},
	year = {2025},
	month = {SEP },
	pages = {125896},
	type = {Article},
	abstract = {<p>
	Wound healing materials with advanced properties that facilitates higher collagen deposition, improved angiogenesis and quick tissue regeneration are crucial for clinical wound management. To meet the requirement, for the first time, our studies focus on engineering bio-originated natural materials, which are tested in combination with the active agents, ascorbic acid (AA), a stimulant and metronidazole (Mtz), an anti-microbial drug. Accordingly, a dual drug (AA, and Mtz) loaded film of functionalized egg albumin (FEA)-poly(vinyl alcohol) (PVA) was fabricated following the solution casting method. The film was characterized for its morphology and physicochemical properties using various analytical tools. The potential of the film as a wound healing material was evaluated, by in vitro drug release, degradation, cell viability, antimicrobial studies, in vivo wound healing, and histopathological analyses. In vitro degradation studies confirmed their degradability in enzymatic and soil burial conditions. Cytotoxicity studies demonstrated their non-toxicity, and the antimicrobial investigations showcased that the material was antibacterial. On the 14th day, the wound closure percentage of the wound induced control group, GI (without treatment) was notably higher at 95 \% compared to the test formulation group, GV [FEA-PVA (30/70 w/w) loaded with Mtz and AA (10 \% w/w of the total polymer weight), respectively], which exhibited a wound closure of 83 \%. Furthermore, the histopathological examinations revealed that the inner wound healing in GV was comparatively better than in GI in terms of angiogenesis, epidermal remodeling, higher collagen deposition, coherency, and tissue regeneration. Consequently, the formulated film can be deemed a suitable wound dressing material.</p>
},
	keywords = {collagen, Dual drug release, Functionalized egg albumin, Polymer blend films, Stimulant, wound Healing},
	issn = {0378-5173},
	doi = {10.1016/j.ijpharm.2025.125896},
	author = {Gautam, Tripurari Rao and Patel, Pratikshkumar R. and Singam, Amarnath R. and Jagtap, Ashish S. and Desai, Prasad and Gundloori, Rathna V. N. and Pawar, Anil and Dastager, Syed G.}
}
