@article {43493,
	title = {Acetone and methanol fruit extracts of terminalia paniculata inhibit HIV-1 infection in vitro},
	journal = {Natural Product Research},
	volume = {31},
	year = {2017},
	month = {JAN},
	pages = {1468-1471},
	abstract = {In this study, we report the in vitro anti-HIV1 activity of acetone and methanol extracts of fruit of Terminalia paniculata. Cytotoxicity tests were conducted on TZM-bl cells and peripheral blood mononuclear cells (PBMC), the CC50 values of both the extracts were 260g/mL. Using TZM-bl cells, the extracts were tested for their ability to inhibit replication of two primary isolates HIV-1 (X4, Subtype D) and HIV-1 (R5, Subtype C). The activity against HIV-1 primary isolate (R5, Subtype C) was confirmed using activated PBMC and by quantification of HIV-1 p24 antigen. Both the extracts showed anti-HIV1 activity in a dose-dependent manner. The EC50 values of the acetone and methanol extracts of T. paniculata were 10.3g/mL. The enzymatic assays were performed to determine the mechanism of action which indicated that the anti-HIV1 activity might be due to inhibition of reverse transcriptase (77.7\% inhibition) and protease (69.9\% inhibition) enzymes. },
	doi = {10.1080/14786419.2016.1258561},
	author = {Durge, Ankita and Jadaun, Pratiksha and Wadhwani, Ashish and Chinchansure, Ashish A. and Said, Madhukar and Thulasiram, H. V. and Joshi, Swati P. and Kulkarni, Smita S.}
}
@article {49001,
	title = {Antibacterial and anti-HIV metabolites from marine streptomyces albus MAB56 isolated from Andaman and Nicobar Islands, India},
	journal = {Applied Biochemistry and Biotechnology},
	volume = {195},
	year = {2023},
	month = {DEC},
	pages = {7738-7754},
	type = {Article},
	abstract = {<p>
	Marine-derived actinobacteria have tremendous potential to produce novel metabolites with diverse biological activities. The Andaman coast of India has a lot of microbial diversity, but it is still a relatively unknown ecology for isolating novel actinobacteria with beneficial bioactive compounds. We have isolated 568 actinobacterial strains from mangrove rhizosphere sediments and sponge samples. Crude extracts from 75 distinct strains were produced by agar surface fermentation and extracted using ethyl acetate. In the disc diffusion method, 25 actinobacterial strains showed antimicrobial activity; notably, the strain MAB56 demonstrated promising broad-spectrum activity. Strain MAB56 was identified as Streptomyces albus by cultural, microscopic, and molecular methods. Conditions for bioactive metabolites from MAB56 were optimized and produced in a lab-scale fermenter. Three active metabolites (C1, C2, and C3) that showed promising broad-spectrum antimicrobial activity were isolated through HPLC-based purification. Based on the UV, FT-IR, NMR, and LC-MS analysis, the chemical nature of the active compounds was confirmed as 12-methyltetradecanoic acid (C1), palmitic acid (C2), and tridecanoic acid (C3) with molecular formulae C14H28O2, C16H32O2, and C13H26O2, respectively. Interestingly, palmitic acid (C2) also exhibited anti-HIV activity with an IC50 value of \&lt; 1 mu g/ml. Our findings reveal that the actinobacteria from the Andaman marine ecosystems are promising for isolating anti-infective metabolites.</p>
},
	keywords = {actinobacteria, Andaman Islands, anti-HIV, Antibacterial, Bioactive metabolites},
	issn = {0273-2289},
	doi = {10.1007/s12010-023-04493-y},
	author = {Manikkam, Radhakrishnan and Murthy, Sangeetha and Palaniappan, Sivasankar and Kaari, Manigundan and Sahu, Amit Kumar and Said, Madhukar and Ganesan, Vijayalakshmi and Kannan, Sivakumar and Ramasamy, Balagurunathan and Thirugnanasambandan, Somasundaram and Dastager, Syed G. and Hanna, Luke Elizabeth and Kumar, Vanaja}
}
