@article {50015, title = {Comprehensive investigation on statistical approaches and classical strain improvement for penicillin V production by Penicillium rubens BIONCL P45 strain}, journal = {Systems Microbiology and Biomanufacturing }, volume = {5}, year = {2025}, month = {JAN}, pages = {1302 -1327}, type = {Article}, abstract = {

Media engineering and strain improvement are critical aspects of microbial biotechnology playing a vital role in enhancing microbial productivity, and ensuring cost-effective bioprocessing. In this investigation, we optimized the various medium components, nutritional condition, and fermentation parameters for the industrial production of phenoxymethylpenicillin or penicillin V (PenV). We have isolated, characterized\ Penicillium rubens\ BIONCL P45 strain which initially produced 100\ mg/L of PenV. Further, optimization using production medium 4 (PM4) comprising lactose, corn steep solids, sodium sulfate, calcium carbonate, and phenoxy acetic acid lead to a significant increase in production, reaching 430\ mg/L. Further improvements through response surface methodology (RSM) predicted a production of 646\ mg/L, which was experimentally validated at 685\ mg/L. Subsequently, mutagenesis studies using UV (ultraviolet) exposure resulted in the UV-65 mutant, which demonstrated a superior performance, achieving 934\ mg/L, surpassing the parental strain. ​These combined strategies lead to a tenfold increase in PenV titer, highlighting their effectiveness in bioprocess development and industrial-scale antibiotic production.

}, keywords = {Box-Behnken design, Classical strain improvement (CSI), Media optimization, Penicillin V, Penicillium rubens, Response surface methodology}, doi = {10.1007/s43393-025-00377-w}, author = {Sawant, Amol Muralidhar and Vamkudoth, Koteswara Rao} }