@article {49541,
	title = {Aspergillus terreus variant TB21 wet biomass optimized by in-situ transesterification for biodiesel production},
	journal = {AMB Express},
	volume = {15},
	year = {2025},
	month = {FEB},
	pages = {23},
	type = {Article},
	abstract = {<p>
	The oleaginous fungus, Aspergillus terreus when subjected to random chemical mutagenesis led to isolation of TB21 variant with improved lipid content (78.1\%) as compared to wild type (49.8\%). The fungal wet biomass grown on sugarcane bagasse hydrolysate (SCBH) was subjected to one-step in-situ (direct) acid transesterification to optimize its conversion to biodiesel using a 2-level factorial statistical design of experiments. The process optimization revealed that wet biomass and methanol were the most significant factors and in a short reaction time period of 5 min with low methanol: wet biomass ratio (10:1) influenced FAME production Statistical optimization studies showed that TB21 exhibited a higher FAME content of 76.5 and 38.1\% (w/w) from wet and dry biomass, respectively when compared to wild type (48.1 and 24.5\%). FAME productivity (0.55-1 h-1) and a yield (66 gL-1) were achieved when TB21 was grown on SCBH for 120 h at 30 degrees C. The FAME profile from the wet biomass of TB21 grown on SCBH had desirable amounts of saturated (77.7\%), monounsaturated (7.2\%), and polyunsaturated (2.4\%) methyl esters. Physico-chemical properties of TB21-derived biodiesel were determined, namely, density(0.88 g cm-3), kinematic viscosity (4.1 mm s-2), iodine value (96.82), cetane number (55.31), free fatty acid content (0.15\%), total acid number (0.3 NaOH mg g-1), meeting international (ASTM D6751, EN 14214) and Indian (IS 15607) standards. Thus, the direct one-pot in situ transesterification reaction using wet biomass of variant TB21 strain showed improved production and quality of biodiesel with potential large scale application using the low-cost substrate (SCBH).</p>
},
	keywords = {Aspergillus Terreus, Biodiesel, In situ transesterification, Mutant, Statistical optimization, Wet biomass},
	issn = {2191-0855},
	doi = {10.1186/s13568-024-01772-7},
	author = {Bed, Rashmi K. and Kumar, V. Ravi and RaviKumar, Ameeta}
}
