@article {48106,
	title = {Superior processability of Antheraea mylitta silk with cryo-milling: performance in bone tissue regeneration},
	journal = {International Journal of Biological Macromolecules},
	volume = {213},
	year = {2022},
	month = {JUL},
	pages = {155-165},
	type = {Article},
	abstract = {<p>
	Non-mulberry silk polymers have a promising future in biomedical applications. However, the dissolution of nonmulberry silk fiber is a still challenge and this poor processability has limited the use of this material. Here, we report a unique protocol to process the Antheraea mylitta (AM) silk fiber. We have shown that the cryo-milling of silk fiber reduces the beta sheet content by more than 10\% and results in an SF powder that completely dissolves in routine solvents like trifluoroacetic acid (TFA) within few hours to form highly concentrated solutions (\~20 wt \%). Further, these solutions can be processed using conventional processing techniques such as electrospinning to form 3D scaffolds. Bombyx mori (BM) silk was used as a control sample in the study. In-vitro studies were also performed to monitor cell adhesion and proliferation and hMSCs differentiation into osteogenic lineage. Finally, the osteogenic potential of the scaffolds was also evaluated by a 4-week implantation study in rat calvarial model. The in-vitro and in-vivo results show that the processing techniques do not affect the biocompatibility of the material and the AM scaffolds support bone regeneration. Our results, thus, show that cryo-milling facilitates enhanced processability of non-mulberry silk and therefore expands its potential in biomedical applications.</p>
},
	keywords = {Bone tissue engineering, Calvarial defect, hMSCs, Non-mulberry silk fiber, Processing technique},
	issn = {0141-8130},
	doi = {10.1016/j.ijbiomac.2022.05.122},
	author = {Parekh, Nimisha and Bijosh, C. K. and Kane, Kartiki and Panicker, Alaka and Nisal, Anuya and Wangikar, Pralhad and Agawane, Sachin}
}
