@article {45689,
	title = {Stability studies of cuticle degrading and mycolytic enzymes of myrothecium verrucaria for control of insect pests and fungal phytopathogens},
	journal = {Indian Journal of Biotechnology},
	volume = {16  },
	year = {2017},
	month = {JUN},
	pages = {404-412},
	type = {Article},
	abstract = {Myrothecium verrucaria produced extracellularly hydrolytic enzymes which can hydrolyse the insect cuticle as well as fungal cell wall. The addition of polyols, such as glycerol, sorbitol, xylitol (1 M) during ultra -filtration or freeze- drying of enzyme mixture increased the recovery of the enzymes in a concentrated form. Polyols (5 M) increased the temperature and pH stability of the enzymes, in the presence of glycerol chitinase, beta-1,3-glucanase, lipase and protease retained 50-60\% of initial activities at 40 degrees C after 3 h. While xylitol (5 M) was effective in stabilizing activities at pH 5.0 and 7.5 at 25 degrees C for 7 d. In the freeze-dried powder form, > 90\% at 4 degrees C for 1 year and 80-85\% at 25 degrees C for 2 months enzyme activities were retained. The addition of glycerol (1 M) to the enzyme mixture protected enzyme activities under sunlight (60-65\% activity at RT) for 5 d. Because of glycerol (1 M), the efficacy of M verrucaria enzyme preparation to control Helicoverpa armigera infestation in chick pea was increased to 70 +/- 19\%. While without glycerol the efficacy was 55 +/- 23\%. The germination of peanut seeds infected with Sclerotium rolfsii was observed to be increased (70 +/- 5\%) in a pot irrigated with enzyme mixture.},
	author = {Chavan, S. B and Vidhate, R. P. and Kallure, G. S. and Dandawate, N. L. and Khire, J. M. and Deshpande, M. V.}
}
