@inbook {43983,
	title = {Molecular methods for identification of clostridium tetani by targeting neurotoxin},
	booktitle = {Methods Molecular Biology},
	volume = {1600},
	year = {2017},
	pages = {37-47},
	abstract = {Tetanus is a potentially fatal muscle spasm disease. It is an important public health problem, especially in rural/tribal areas of developing countries. Tetanus toxin, a neurotoxin (tetanospasmin ), is the most important virulence factor that plays a key role in the pathogenicity of tetanus . Confirmation of virulence by confirming the production of tetanospasmin by infecting species forms the most important part in the diagnosis of tetanus . Various molecular methods have been devised for confirmation of diagnosis by targeting different genes. The most common molecular methods are tetanospasmin producing (TetX) gene-targeted methods using TetX-specific primers. Here, we describe various molecular methods targeting TetX gene such as polymerase chain reaction, pulsed-field gel electrophoresis, Southern blotting, loop-mediated isothermal amplification assay, etc. to confirm the virulence of Cl. tetani.},
	doi = {10.1007/978-1-4939-6958-6_4},
	author = {Dharne, Mahesh S. and Nagoba, Basavraj S. and Gohil, Kushal N.}
}
