@conference {42952,
	title = {Quantitative, automated, high content screening assay approach to anti-angiogenicdrug discovery},
	booktitle = {National Science Day, At CSIR-NCL},
	year = {2015},
	month = {FEB},
	address = {Combi Chem-Bio Resource Center, OCD, National Chemical Laboratory, Pune - 411 008},
	abstract = {<p>Angiogenesisenhancescancer,maculardegeneration,etc.. Currentangiogenicassays--indirect,manual,timeconsuming,laborintensive,difficulttoquantify,andexpensive. Wedevelopedanautomated,quantitative,HighContentScreeningangiogenictubeformationassaywouldbenefitdrugdiscoveryforseveraltherapeuticareas. Humanendothelialcellswereseededwithpro-oranti-angiogeniccompoundsonaMatrigelTMlayerina96-wellplate.Afterangiogenictubesformation,thepositivecontrolsandthetestsampleswerefixed,fluorescentlylabeled,automaticallyimagedonCellomics{\textquoteright}ArrayScan{\textregistered}HCSReader,andquantitativelyanalyzedon-the-flybytheTubeFormationBioApplicationsoftwaremodule. Multiplefeaturesrelevantforangiogenesisandangiogenictubeformationwereautomaticallymeasured. Thisrobustandreliableassaythusenabledthelarge-scale,quantitative,screeningandcharacterizationofcompoundsaffectingbothpro-andanti-angiogenicpathways.</p>},
	author = {Nawale, Laxman and Sarkar, Dhiman}
}
