@article { ISI:000243627100004,
	title = {Attempt at taxonomical characterization of some Rhizobial species by intact cell MALDI mass spectrometry},
	journal = {World Journal of Microbiology \& Biotechnology},
	volume = {23},
	number = {2},
	year = {2007},
	month = {FEB},
	pages = {177-185},
	publisher = {SPRINGER},
	type = {Article},
	address = {233 SPRING STREET, NEW YORK, NY 10013 USA},
	abstract = {<p>In the present study an attempt was made to exploit the benefit of intact cell MALDI mass spectrometry (ICM-MS) in bringing out similarities and differences among some Rhizobium species and a species of Agrobacterium based on specific mass:charge (m/z) values. Rhizobium species isolated from the root nodules of selected leguminous plants were analysed by ICM-MS. The spectra were acquired in the range of 500-10,000 Da yielding several peaks specific to each species. The peaks obtained corresponded to the respective bacterial cell surface molecules which were desorbed during matrix-assisted laser desorption ionization. The number of peaks were more in the range of 500-1200 Da. Dice similarity coefficient analysis of m/z values indicated that Rhizobium species isolated from Trigonella foenum-graecum and Pisum arvense showed more similarity than any other species. Agrobacterium species did show a few common m/z values in comparison with other Rhizobium species. This clearly shows that Agrobacterium is closely related to Rhizobium. Eventually, ICM-MS technique offers clear, distinct, and consistent results for replicates, in less than an hour{\textquoteright}s time, therefore this technique has high potential in molecular taxonomy.</p>
},
	keywords = {ICM-MS, legume, molecular taxonomy, Rhizobium, symbiotic},
	issn = {0959-3993},
	doi = {10.1007/s11274-006-9207-4},
	author = {Prabhu, R. R. and Kulkarni, Mahesh J. and Parasharami, V. A. and Santhakumari, B. and Paranjape, S.}
}
