@article { ISI:000254833600003,
	title = {Media optimization for the production of cephalosporin C acylase from a novel bacterial source: alcaligenes xylosoxidans MTCC*491},
	journal = {Research Journal of Biotechnology},
	volume = {3},
	number = {1},
	year = {2008},
	month = {FEB},
	pages = {16-21},
	publisher = {RESEARCH JOURNAL BIOTECHNOLOGY},
	address = {SECTOR A-80, SCHEME NO 54, VIJAY NAGAR, A B ROAD, INDORE, 452 010 MP, INDIA},
	abstract = {<p>The Gram-negative bacterium Alcaligenes xylosoxidans MTCC *491 has been studied for the production of cephalosporin C acylase (CCA), a pharmaceutically important enzyme. The enzyme hydrolyses cephalosporin C (CPC), a natural product, to 7-aminocephalosporanic acid, a key intermediate in the production of semisynthetic cephalosporin antibiotics. Effect of medium, pH, temperature, carbon source, nitrogen source and incubation time, on the production of CCA was studied. 7.7 IU/ml activity was obtained when Luria Bertani broth (LB) was used as a fermentation medium. Glucose and galactose increased the enzyme activity by 9 \% and 22 \%, while all the nitrogen sources tested showed a repressive effect. Maximum CCA activity was observed around the 5(th) day of incubation indicating potential role of CCA in secondary metabolism. pH 8.0 and 40 degrees C were found to be the optimum conditions for acylase activity.</p>},
	keywords = {alcaligenes xylosoxidans MTCC*491, cephalosporin C (CPC), cephalosporin C acylase (CCA), fermentation carbon source, nitrogen source},
	issn = {0973-6263},
	author = {Nupura, Hirve and Asmita, Tingare and Sharat, B. and Asmita, Prabhune}
}
