@article { ISI:000256285600005,
	title = {Studies on optimization of cultural conditions and effect of media constituents on production of intracellular penicillin V acylase from Erwinia aroideae (DSMZ 30186)},
	journal = {Research Journal of Biotechnology},
	volume = {3},
	number = {2},
	year = {2008},
	month = {MAY},
	pages = {26-30},
	publisher = {RESEARCH JOURNAL BIOTECHNOLOGY},
	address = {SECTOR A-80, SCHEME NO 54, VIJAY NAGAR, A B ROAD, INDORE, 452 010 MP, INDIA},
	abstract = {<p>Penicillin V acylase (PVA) is a pharmaceutically important enzyme. Erwinia aroideae (DSMZ 30186) produced high levels of intracellular penicillin V acylase. Amongst various carbon sources used (2.0 \%); fructose, galactose, sucrose and mannitol increased enzyme production up to 579, 544, 534 and 504 IU/g dry weight (DW), respectively, compared to minimal media (263 IU/g DW); however maximum PVA productivity (2796 IU/ L) was achieved using fructose. Ammonium ions escalated the enzyme production to 1266.9 IU/g DW; however sodium glutamate was the best nitrogen source for overall productivity of enzyme (2045 IU/ L). Cornsteep liquor and skim milk were used as supplements; 1.0 \% cornsteep liquor and 3.0 \% skim milk were optimum for the production of PVA up to 823 and 93 IU/g DW, respectively. Optimum cultural conditions for the production of PVA from E. aroideae were standardized. Erwinia aroideae produced 1543 IU/g DW penicillin V acylase in Erlenmeyer flasks (250 ml) containing 50 ml of minimal medium with 0.3\% ammonium sulphate, pH 7. 0 at 28 degrees C and 180 rpm when incubated for 56 hr.</p>},
	keywords = {carbon sources, Erwinia aroideae, intracellular, nitrogen sources, Penicillin V acylase},
	issn = {0973-6263},
	author = {Atul, Kumar and Vivek, Lonkar and Archana, Pundle}
}
