@article { ISI:000267914600004,
	title = {Identification of Indian pathogenic races of fusarium oxysporum f. sp ciceris with gene specific, ITS and random markers},
	journal = {Mycologia},
	volume = {101},
	number = {4},
	year = {2009},
	month = {AUG},
	pages = {484-495},
	publisher = {ALLEN PRESS INC},
	address = {810 E 10TH ST, LAWRENCE, KS 66044 USA},
	abstract = {<p>In this study we demonstrate the synergistic use of gene-specific markers, ITS-RFLP, ISSR and AFLP for distinguishing Indian F. oxysporum f. sp. ciceris races. We also report for the first time that F. oxysporum f. sp. ciceris race 3, a wilt pathogen of chickpea in India, is actually E proliferatum based on phylogenetic analysis with EF-1 alpha sequence data. E oxysporum f. sp. ciceris races 1, 2 and 4 were easily distinguished from {\textquoteleft}{\textquoteleft}race 3{\textquoteright}{\textquoteright} (F. proliferatum) by PCR amplification with oligonucleotides designed from conserved regions of Hop78 transposon (Hop 78), cutinase (Cut), desaturase (Dst). F oxysporum f. sp. ciceris race 4 was distinguished with the xylanase 3 (xyl3) gene by absence of amplification product only in this race. The Xyl3 amplified-DNA fragment isolated and sequenced from E oxysporum f. sp. ciceris race I was similar to the F-xylanase (Xyl3) gene of E oxysporum f. sp. lycopersici. A TELD motif, which is characteristic of the F-xylanases family, was detected within the deduced amino acid sequence of F. oxysporum f. sp. ciceris. Similarly the F oxysporum f. sp. ciceris Hop78 DNA fragment, which identified {\textquoteleft}{\textquoteleft}race 3{\textquoteright}{\textquoteright} (E proliferatum), was homologous to the Hop78 transposon of E oxysporum f. sp. melonis, including the 100 amino acid conserved domain and the characteristic CCHC motif. The internal transcribed spacer region-restriction fragment length polymorphism (ITS-RFLP) approach along with intersimple sequence repeat (ISSR) method also differentiated {\textquoteleft}{\textquoteleft}race 3{\textquoteright}{\textquoteright} (F. proliferatum). Races 1 and 2 were identified by unique AFLP patterns. Sequence characterization of race-specific AFLP products revealed significant homologies of these sequences with metabolically important genes.</p>},
	keywords = {AFLP, F. oxysporum f. sp ciceris races, gene specific amplification, ISSR, ITS-RFLP, race delineation, sequence characterization, translation elongation factor (EF-1 alpha)},
	issn = {0027-5514},
	doi = {10.3852/08-085},
	author = {Gurjar, Gayatri and Barve, Maneesha and Giri, Ashok P. and Gupta, Vidya}
}
