@article { ISI:000293993600017,
	title = {Method to extract intact and pure RNA from mycobacteria},
	journal = {Analytical Biochemistry},
	volume = {417},
	number = {2},
	year = {2011},
	month = {OCT},
	pages = {286-288},
	publisher = {ACADEMIC PRESS INC ELSEVIER SCIENCE},
	address = {525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA},
	abstract = {<p>We describe a high-yielding, simple, and aerosol-free protocol for the isolation of RNA from mycobacteria that does not require sophisticated instruments. The method yielded 50 mu g of RNA from 10(7) cells, 50 times more than a recently reported method. Our method can extract total RNA from aerobically grown bacteria and from in vitro hypoxia-induced dormant bacilli and mycobacteria residing within infected macrophages. (C) 2011 Elsevier Inc. All rights reserved.</p>},
	keywords = {Dormant, Hypoxia, Mycobacteria, RNA isolation, Spheroplast, Trizol},
	issn = {0003-2697},
	doi = {10.1016/j.ab.2011.06.028},
	author = {Akhtar, Shamim and Sarkar, Sampa and Mishra, Abhishek and Sarkar, Dhiman}
}
