@article { ISI:000303951600008,
	title = {Controlled synthesis of O-glycopolypeptide polymers and their molecular recognition by lectins},
	journal = {Biomacromolecules},
	volume = {13},
	number = {5},
	year = {2012},
	month = {MAY},
	pages = {1287-1295},
	publisher = {AMER CHEMICAL SOC},
	type = {Article},
	address = {1155 16TH ST, NW, WASHINGTON, DC 20036 USA},
	abstract = {<p>The facile synthesis of high molecular weight water-soluble O-glycopolypeptide polymers by the ring-opening polymerization of their corresponding N-carboxyanhydride (NCA) in very high yield (overall yield \&gt; 70\%) is reported. The per-acetylated-O-glycosylated lysine-NCA monomers, synthesized using stable glycosyl donors and a commercially available protected amino acid in very high yield, was polymerized using commercially available amine initiators. The synthesized water-soluble glycopolypeptides were found to be alpha-helical in aqueous solution. However, we were able to control the secondary conformation of the glycopolypeptides (alpha-helix vs nonhelical structures) by polymerizing raceznic amino acid glyco NCAs. We have also investigated the binding of the glycopolypeptide poly(alpha-manno-O-lys) with the lectin Con-A using precipitation and hemagglutination assays as well as by isothermal titration calorimetry (ITC). The ITC results clearly show that the binding process is enthalpy driven for both alpha-helical and nonhelical structures, with negative entropic contribution. Binding stoichiometry for the glycopolypeptide poly(alpha-manno-O-lys) having a nonhelical structure was slightly higher as compared to the corresponding polypeptide which adopted an alpha-helical structure.</p>},
	issn = {1525-7797},
	doi = {10.1021/bm201813s},
	author = {Pati, Debasis and Shaikh, Ashif Y. and Das, Soumen and Nareddy, Pavan Kumar and Swamy, Musti J. and Hotha, Srinivas and Sen Gupta, Sayam}
}
