@article { ISI:000306585900004,
	title = {Production, purification, and characterization of a beta-glucosidase of penicillium funiculosum NCL1},
	journal = {Applied Biochemistry and Biotechnology},
	volume = {167},
	number = {5},
	year = {2012},
	month = {JUL},
	pages = {959-972},
	publisher = {HUMANA PRESS INC},
	address = {999 RIVERVIEW DRIVE SUITE 208, TOTOWA, NJ 07512 USA},
	abstract = {<p>Penicillium funiculosum NCL1, a filamentous fungus, produced significantly higher levels of beta-glucosidase. The effect of initial pH, incubation temperature, and different carbon sources on extracellular beta-glucosidase production was studied in submerged fermentation. At 30 A degrees C with initial pH 5.0, enzyme production was increased by 48-fold upon induction with paper mill waste, as compared to commercial cellulose powder. In zymogram analysis, four isoforms of beta-glucosidases were observed with wheat bran whereas a minimum of one isoform was observed with other carbon sources. A major beta-glucosidase (Bgl3A) with the apparent molecular weight of similar to 120 kDa, induced by paper mill waste, was purified 19-fold to homogeneity, with a specific activity of 1,796 U/mg. Bgl3A was a monomeric glycoprotein with 29\% of neutral carbohydrate content. It showed optimum activity at pH 4.0 and 5.0, optimum temperature at 60 A degrees C, and exhibited a half-life of 1 h at 60 A degrees C. K (m) of Bgl3A was found to be 0.057 mM with p-nitrophenyl beta-d-glucoside and V (max) was 1,920 U/mg. The purified enzyme exhibited glucose tolerance with a K (i) of 1.5 mM. Bgl3A readily hydrolyzed glucosides with beta-linkage. Bgl3A activity was enhanced (156\%) by Zn2+ and was not affected by other metal cations and reagents. The supplementation of Bgl3A (5 U/mg) with Trichoderma reesei cellulase complex (5 FPU/mg) resulted in about 70\% of enhanced glucose production, which emphasizes the industrial importance of Bgl3A.</p>},
	keywords = {beta-Glucosidase, Paper mill waste, Penicillium funiculosum NCL1, Purification, Thermostability},
	issn = {0273-2289},
	doi = {10.1007/s12010-012-9645-4},
	author = {Ramani, Gurusamy and Meera, Balasubramanian and Vanitha, Chinnathambi and Rao, Mala and Gunasekaran, Paramasamy}
}
